Methods and systems for genetic analysis
View Patent ↗This disclosure provides systems and methods for sample processing and data analysis. Sample processing may include nucleic acid sample processing and subsequent sequencing. Some or all of a nucleic acid sample may be sequenced to provide sequence information, which may be stored or otherwise maintained in an electronic storage location. The sequence information may be analyzed with the aid of a computer processor, and the analyzed sequence information may be stored in an electronic storage location that may include a pool or collection of sequence information and analyzed sequence information generated from the nucleic acid sample. Methods and systems of the present disclosure can be used, for example, for the analysis of a nucleic acid sample, for producing one or more libraries, and for producing biomedical reports. Methods and systems of the disclosure can aid in the diagnosis, monitoring, treatment, and prevention of one or more diseases and conditions.
1. A method for analyzing a nucleic acid sample, comprising:
a. producing two or more subsets of nucleic acid molecules from a nucleic acid sample, wherein the two or more subsets differ by one or more genomic features selected from the group consisting of: genomic regions, mean GC content, mean molecular size, methylation state, insertion or deletion, and single nucleotide variant (SNV), with the aid of pulldown probes that selectively enrich for the one or more genomic features in at least a first subset or a second subset of the two or more subsets of nucleic acid molecules as compared to another genomic feature(s) in the at least the first subset or the second subset;
b. combining at least two of the two or more subsets of nucleic acid molecules to produce a first combined pool of nucleic acid molecules; and
c. conducting one or more assays on the first combined pool of nucleic acid molecules, wherein at least one of the one or more assays comprises a sequencing reaction.
2. The method of claim 1 , wherein the nucleic acid molecules are DNA.
3. The method of claim 1 , wherein the genomic regions are selected from the group consisting of alternative sequences, entire exome, a set of genes, and a set of regulatory elements.
4. The method of claim 3 , wherein the set of genes is selected from the group consisting of a set of genes with Mendelian traits, a set of genes with disease traits, a set of genes with known drug traits, and a set of genes with biomedically interpretable variants.
5. The method of claim 3 , wherein the alternative sequences are selected from the group consisting of one or more small insertions, small deletions, structural variant junctions, variable length tandem repeats, and flanking sequences.
6. The method of claim 1 , wherein the two or more subsets of nucleic acid molecules differ by mean molecular size.
7. The method of claim 1 , further comprising producing a unified assessment of a genetic state of the nucleic acid sample at each locus addressed by the one or more assays.
8. The method of claim 1 , wherein conducting the one or more assays further comprises conducting one or more amplification reactions.
9. The method of claim 8 , wherein conducting the one or more amplification reactions comprises polymerase chain reaction-based amplification.
10. The method of claim 1 , wherein producing two or more subsets of nucleic acid molecules from a nucleic acid sample with the aid of pulldown probes comprises conducting one or more hybridization reactions.
11. The method of claim 1 , wherein producing the two or more subsets further comprises differential amplification of the two or more subsets based on the one or more genomic features.
12. The method of claim 1 , wherein conducting one or more assays comprises conducting two or more assays, and further comprising (d) combining results from the two or more assays.
13. The method of claim 1 , further comprising producing one or more biomedical reports after conducting the one or more assays, wherein the one or more biomedical reports comprise biomedical information of a subject from which the nucleic acid sample was derived.
14. The method of claim 13 , wherein the biomedical information of the subject is indicative of one or more biomedical features selected from the group consisting of disease state, genetic risk of a disease, reproductive risk, genetic risk to a fetus, risk of an adverse drug reaction, efficacy of a drug therapy, prediction of optimal drug dosage, and transplant tolerance.
15. The method of claim 1 , wherein the first subset of nucleic acid molecules and the second subset of nucleic acid molecules of the two or more subsets of nucleic acid molecules are complementary with respect to the one or more genomic features selected from the group consisting of genomic regions, mean GC content and mean molecular size.
16. The method of claim 1 , wherein nucleic acid molecules of the two or more subsets differ at least by mean GC content.
17. The method of claim 1 , wherein nucleic acid molecules of the two or more subsets differ by at least mean GC content and mean molecular size.