Recovery and processing of human embryos formed in vivo
A kit having instructions for use for performing uterine lavage in a female patient includes a uterine lavage catheter configured for insertion into a woman's uterus to remove viable blastocysts from the uterus, and one or more first containers having a sufficient dosage amount of a GnRH antagonist to cause desynchronization of the endometrium of the patient prior to, during and/or following recovery of viable blastocysts from the uterus.
1. A kit for uterine lavage, consisting of:
a uterine lavage catheter configured for insertion into a woman's uterus to remove blastocysts from the uterus; and
one or more first containers with a sufficient dosage amount of a GnRH antagonist to cause corpus luteum apoptosis leading to desynchronization of the endometrium of the uterus.
2. A kit for uterine lavage, consisting of:
a uterine lavage catheter configured for insertion into a woman's uterus;
one or more first containers with a sufficient dosage amount of a GnRH antagonist to cause corpus luteum apoptosis leading to desynchronization of the endometrium of the uterus; and
a cell-culture container for collecting lavage fluid from the lavage catheter.
3. A kit for uterine lavage, consisting of:
a uterine lavage catheter configured for insertion into a woman's uterus;
one or more first containers with a sufficient dosage amount of a GnRH antagonist to cause corpus luteum apoptosis leading to desynchronization of the endometrium of the uterus; and
a lavage controller for controlling the delivery of pressure and the application of vacuum to the lavage catheter.
4. A kit for uterine lavage, consisting of:
a uterine lavage catheter configured for insertion into a woman's uterus;
one or more first containers with a sufficient dosage amount of a GnRH antagonist to cause corpus luteum apoptosis leading to desynchronization of the endometrium of the uterus; and
a programmable lavage controller for automatically controlling the delivery of pressure and the application of vacuum to the lavage catheter.
5. A kit for uterine lavage, consisting of:
a uterine lavage catheter configured for insertion into a woman's uterus for removal of one or more blastocysts from the uterus;
one or more first containers with a sufficient dosage amount of a GnRH antagonist to cause corpus luteum apoptosis leading to desynchronization of the endometrium of the uterus; and
a cell transport vial for transport of lavage fluid containing the one or more blastocysts to a laboratory.
6. A kit for uterine lavage, consisting of:
a uterine lavage catheter configured for insertion into a woman's uterus for removal of one or more blastocysts from the uterus;
one or more first containers with a sufficient dosage amount of a GnRH antagonist to cause corpus luteum apoptosis leading to desynchronization of the endometrium of the uterus; and
one or more of a: (i) cell culture container for maintaining blastocyst-safe 02/CO2 levels without the requirement of an incubator, (ii) a cell transport vial for transport of lavage fluid containing the one or more blastocysts to a laboratory, (iii) a lavage controller for controlling the delivery of pressure and the application of vacuum to the lavage catheter, and (iv) a programmable controller for automatically controlling the delivery of pressure and the application of vacuum to the lavage catheter.