Respiratory syncytial virus with a genomic deficiency complemented in trans
The invention relates to pneumoviral virions comprising a viral genome that has a mutation in a gene coding for a protein that is essential for infectivity of the pneumovirus, whereby the mutation causes a virus produced from only the viral genome to lack infectivity, and whereby the virion comprises the protein in a form and in an amount that is required for infectivity of the virion. The invention also relates to methods for producing the pneumoviral virions and for using the virions in the treatment or prevention of pneumoviral infection and disease. A preferred pneumoviral virion is a virion of Respiratory Syncytial Virus in which preferably the gene for the G attachment protein is inactivated and complemented in trans.
1. A vaccine composition consisting essentially of:
(a) a virion of a human Respiratory Syncytial Virus comprising a viral genome that has an inactivating mutation in only the gene coding for a G attachment protein;
(b) an adjuvant; and, optionally,
(c) a pharmaceutically acceptable excipient selected from the group consisting of carriers, stabilisers, solubilisers, and preservatives.
2. The vaccine composition of claim 1 , wherein the inactivating mutation comprises a deletion of the entire sequence coding for the G attachment protein from the viral genome.
3. The vaccine composition according to claim 1 , wherein the carrier is selected from water, buffered saline solutions, glycerin, polysorbate 20, cremophor EL, and an aqueous mixture of caprylic/capric glyceride.