Plant regulatory sequence
View Patent ↗The present invention relates to regulatory sequences. In particular, the invention relates to a regulatory nucleic acid molecule, at least part of which has a transcription initiation function directing expression of an operably associated protein encoding polynucleotide of interest to non-tassel tissue in maize, but not or substantially not to tassel. The invention further relates to chimeric genes and expression cassettes comprising the regulatory nucleic acid molecule and to transgenic plants comprising the chimeric genes and expression cassettes.
1. An isolated polynucleotide comprising SEQ ID NO:35 operably linked to a heterologous nucleotide sequence of interest in maize, wherein the heterologous nucleotide sequence is transcribed in maize leaf tissue and not in tassel.
2. An expression cassette comprising the isolated polynucleotide of claim 1 .
3. A vector molecule comprising the expression cassette according to claim 2 .
4. A transgenic plant comprising the expression cassette of claim 2 .
5. A transgenic plant comprising the vector of claim 3 .
6. The transgenic plant of claim 5 wherein the plant is Zea mays.
7. A seed of the transgenic plant of claim 6 , wherein the seed comprises an isolated polynucleotide comprising SEQ ID NO: 35 operably linked to a heterologous nucleotide sequence of interest in maize.