Methods and compositions to aggregate algae
View Patent ↗The present invention comprises methods and compositions for aggregating algae so as to separate the algal cells from an aqueous algae suspension. A bioflocculent, comprising a composition comprising a bacteria, is used to aggregate the algae.
1. A method for the separation of microalgae from an aqueous suspension of microalgae, while maintaining the integrity of the cell structure, comprising,
a) adding to an aqueous suspension of microalgae a bioflocculating bacteria, causing flocculation of at least some of the microalgae cells, wherein the bioflocculating bacteria is Paenibacillus polymyxa Strain 2 deposited with the American Type Culture Collection (ATCC) under Accession Number PTA-12841; and
b) after at least a portion of the microalgae is flocculated, removing at least a portion of the liquid; wherein at the same time of, or before, adding the Paenibacillus polymyxa Strain 2 in a), discontinuing providing additional nutrient media to the aqueous suspension of microalgae.
2. The method of claim 1 , wherein at least some of the microalgae flocculated are from the genera of Chlorella, Scenedesmus, Nannochloris, Chlamydomonas, Chlorococcum, Cryptomonas, Microactinium or Ellipsoidion.
3. The method of claim 1 , wherein the time for flocculating the microalgae is at least 2 days.
4. The method of claim 1 , wherein the time for flocculating the microalgae is at least 4 days.
5. The method of claim 1 , wherein the time for flocculating the microalgae is 5 days.
6. The method of claim 1 , wherein the microalgae suspension is contained in a pond of at least 60,000 gallons.
7. The method of claim 1 , wherein the bioflocculating bacteria is added by spraying or sprinkling the bacteria on the surface of the microalgae suspension.
8. The method of claim 1 , further comprising c) removing at least a portion of the flocculated microalgae.
9. The method of claim 8 , wherein steps b) and c) can be performed in any order.