DIG-17 insecticidal cry toxins
View Patent ↗DIG-17 insecticidal toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.
1. A pesticide formulation comprising a pesticidal effective amount of DIG-17 insecticidal toxin polypeptide having growth inhibitory activity against Coleopteran insects, wherein the polypeptide comprises a core toxin segment that includes residues 2 to 666 of SEQ ID NO:5 linked to a C-terminal protoxin portion of a Cry toxin other than DIG-17.
2. The pesticide formulation of claim 1 , wherein the polypeptide is a chimeric protein and the C-terminal protoxin portion of cry1Ab or a cry1Ac/cry1Ab chimeric toxin.
3. The pesticide formulation of claim 2 , wherein the C-terminal protoxin portion comprises the C-terminal protoxin portion of Cry1Ab.
4. The pesticide formulation of claim 3 , wherein the C-terminal protoxin portion comprises the C-terminal protoxin portion of cry1Ac/cry1Ab chimeric toxin.
5. A method for controlling a Coleopteran pest population comprising contacting said population with a pesticidally effective amount of a DIG-17 insecticidal toxin polypeptide having growth inhibitory activity against Coleopteran insects, wherein the polypeptide comprises a core toxin segment that includes residues 2 to 666 of SEQ ID NO:5.
6. The method of claim 5 , wherein the pest population is a Colorado potato beetle population.
7. A nucleic acid construct, wherein the construct comprises a heterologous nucleic acid sequence that is recombinantly linked to a sequence encoding a DIG-17 insecticidal toxin polypeptide having growth inhibitory activity against Coleopteran insects, wherein the polypeptide comprises a core toxin segment that includes residues 2 to 666 of SEQ ID NO:5, and wherein the heterologous nucleic acid sequence is a promoter, enhancer, multiple cloning site, or vector sequence, or protoxin portion of a Cry toxin other than DIG-17.
8. The nucleic acid construct of claim 7 , wherein the heterologous nucleic acid sequence is a promoter sequence capable of driving expression in a plant and the promoter is operably linked to the sequence encoding the DIG17 insecticidal toxin polypeptide.
9. The nucleic acid construct of claim 8 , wherein the sequence encoding the core toxin segment is codon-optimized for expression in a plant.
10. The nucleic acid construct of claim 9 , wherein the promoter is capable of driving expression in corn and the sequence encoding the DIG-17 core toxin segment is codon optimized for expression in corn.
11. The nucleic acid construct of claim 7 , wherein the sequence encoding the polypeptide comprises SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, or SEQ ID NO:6.
12. The nucleic acid construct of claim 11 , wherein the construct is a vector.
13. The nucleic acid construct of claim 11 , wherein the construct is a vector and the nucleic acid sequence encoding the core toxin segment is linked to a sequence encoding C-terminal protoxin portion of a Cry toxin other than DIG-17.
14. The nucleic acid construct of claim 13 , wherein the nucleic acid sequence encoding the core toxin segment is linked to a sequence encoding a C-terminal protoxin portion of Cry1Ab or a C-terminal protoxin portion of cry1Ac/cry1Ab chimeric toxin.
15. The nucleic acid construct of claim 9 , wherein the construct comprises a promoter and the promoter is capable of driving expression in potato and the sequence encoding the polypeptide is codon optimized for expression in potato.
16. A transgenic plant comprising the nucleic acid construct of claim 7 .
17. A transgenic plant comprising the nucleic acid construct of claim 11 .