Adherent cells from placenta and use of same in disease treatment
Methods for treating conditions by administration of placenta derived adherent stromal cells to a subject in thereof are provided. Such conditions include skeletal muscle defects, neuropathic pain, and myocardial infarction. Also provided are methods wherein the adherent stromal cells administered are cultured under 2 dimensional or 3 dimensional growth conditions. Also provided are methods in which the cells administered are at least 70% adherent cells from a maternal or fetal portion of the placenta.
1. A method of treating skeletal muscle trauma resulting directly from a physical injury in a subject in need thereof, the method comprising administering to said subject a composition comprising a population of adherent cells derived from a placenta, wherein the population comprises adherent cells from a maternal portion of the placenta, thereby treating the skeletal muscle trauma.
2. The method of claim 1 , wherein said adherent cells are less committed to differentiation into osteogenic lineages as compared to adherent cells from bone marrow grown and differentiated under the same conditions.
3. The method of claim 1 , wherein said adherent cells are less committed to differentiation into adipogenic lineages as compared to adherent cells from bone marrow grown and differentiated under the same conditions.
4. The method of claim 1 , wherein said adherent cells express one or more of CD73, CD90, CD29, CD105 or D7-fib.
5. The method of claim 1 , wherein said adherent cells from the maternal portion of the placenta do not express CD3, CD4, CD45, CD80, HLA-DR, CD11b, CD14, CD19, CD34, CD31, CD200, KDR, or CD79.
6. The method of claim 1 , wherein said adherent cells express one or more of beta-Endorphin, dynorphin A, leu-enkephalin, or met-enkephalin.
7. The method of claim 1 , wherein the physical injury is a surgical injury.
8. The method of claim 1 , wherein the physical injury is an orthopedic injury.
9. The method of claim 1 , wherein said adherent cells do not differentiate into osteogenic lineages under conditions in which bone marrow-derived mesenchymal stem cells differentiate into osteogenic lineages.
10. The method of claim 9 , wherein said adherent cells do not differentiate into adipogenic lineages under conditions in which bone marrow-derived mesenchymal stem cells differentiate into adipogenic lineages.
11. The method of claim 1 , wherein said adherent cells do not differentiate into adipogenic lineages under conditions in which bone marrow-derived mesenchymal stem cells differentiate into adipogenic lineages.
12. A method of treating skeletal muscle trauma in a subject in need thereof, the method comprising administering to said subject a composition comprising a population of adherent cells derived from a placenta, wherein the population comprises adherent cells from a maternal portion of the placenta, and wherein said skeletal muscle trauma does not result directly from ischemia, thereby treating the skeletal muscle trauma.
13. The method of claim 12 , wherein said adherent cells are less committed to differentiation into osteogenic lineages as compared to adherent cells from bone marrow grown and differentiated under the same conditions.
14. The method of claim 12 , wherein said adherent cells are less committed to differentiation into adipogenic lineages as compared to adherent cells from bone marrow grown and differentiated under the same conditions.
15. The method of claim 12 , wherein said adherent cells express one or more of CD73, CD90, CD29, CD105 or D7-fib.
16. The method of claim 12 , wherein said adherent cells from the maternal portion of the placenta do not express CD3, CD4, CD45, CD80, HLA-DR, CD11b, CD14, CD19, CD34, CD31, CD200, KDR, or CD79.
17. The method of claim 12 , wherein said adherent cells express one or more of beta-Endorphin, dynorphin A, leu-enkephalin, or met-enkephalin.
18. The method of claim 12 , wherein said adherent cells do not differentiate into osteogenic lineages under conditions in which bone marrow-derived mesenchymal stem cells differentiate into osteogenic lineages.
19. The method of claim 18 , wherein said adherent cells do not differentiate into adipogenic lineages under conditions in which bone marrow-derived mesenchymal stem cells differentiate into adipogenic lineages.
20. The method of claim 12 , wherein said adherent cells do not differentiate into adipogenic lineages under conditions in which bone marrow-derived mesenchymal stem cells differentiate into adipogenic lineages.