Fluid cartridge

1. A method for transferring a liquid from a fluid cartridge comprising a casing having a top surface, a fluid chamber comprising a well formed in the casing with an opening at the top surface that is sealed to prevent or limit liquid evaporation through the opening, a fluid reservoir adjacent to the fluid chamber and comprising a well formed in the casing with an opening at the top surface that is sealed to prevent or limit liquid evaporation through the opening, wherein the fluid chamber and the fluid reservoir contain the same liquid, wherein the opening of the fluid reservoir is larger than the opening of the fluid chamber, and wherein the opening of the fluid chamber is sealed by a frangible seal secured to the top surface and covering the opening of the fluid chamber, a first path between the fluid reservoir and the fluid chamber disposed proximate a lower portion of the fluid reservoir and fluid chamber and providing liquid communication between the fluid reservoir and the fluid chamber, and a second path between the fluid reservoir and fluid chamber disposed proximate a top portion of the fluid reservoir and fluid chamber and providing fluid communication between the fluid reservoir and the fluid chamber, the method comprising:

(a) penetrating the frangible seal covering the opening of the fluid chamber with an automated pipettor;

(b) contacting the liquid contained in the fluid chamber with the automated pipettor; and

(c) withdrawing at least a portion of the liquid from the fluid chamber with the automated pipettor, thereby causing liquid to flow from the fluid reservoir into the fluid chamber through the first path while air flows from the fluid chamber to the fluid reservoir through the second path.

2. The method of claim 1 , wherein the frangible seal covering the opening of the fluid chamber comprises a laminate having a foil layer.

3. The method of claim 2 , wherein the laminate further includes a plastic liner.

4. The method of claim 1 , wherein the automated pipettor includes a removably attached pipette tip, and wherein step (a) is performed by puncturing that portion of the frangible seal which covers the opening of the fluid chamber with the pipette tip and step (c) comprises drawing at least a portion of the liquid into the pipette tip.

5. The method of claim 1 , further comprising, after step (c), moving the automated pipettor to a well and then dispensing at least a portion of the liquid withdrawn from the fluid chamber in step (c) into the well.

6. The method of claim 5 , further comprising penetrating a frangible seal covering the well with the automated pipettor prior to dispensing at least a portion of the liquid into the well.

7. The method of claim 5 , wherein the well is part of a tray having one or more wells, and wherein the method further comprises releasably securing the fluid cartridge and the tray to a rack configured to be inserted into an automated analyzer.

8. The method of claim 1 , wherein the liquid comprises a reconstitution reagent, and the method further comprises, after step (c), moving the automated pipettor to a reagent well containing a lyophilized reagent and then dispensing at least a portion of the liquid drawn from the fluid chamber in step (c) into the reagent well to form a reconstituted reagent.

9. The method of claim 8 , further comprising alternately aspirating the liquid from and dispensing the liquid into the reagent well one or more times.

10. The method of claim 8 , further comprising withdrawing at least a portion of the reconstituted reagent from the reagent well with the automated pipettor, moving the automated pipettor to a receptacle, and dispensing at least a portion of the reconstituted reagent into the receptacle with the automated pipettor.

11. The method of claim 10 , further comprising alternately aspirating the liquid from and dispensing the liquid into the receptacle one or more times.

12. The method of claim 10 , wherein the method further comprises, after dispensing the reconstituted reagent into the receptacle, picking up a cap with a robot arm and attaching the cap to the receptacle with the robot arm.

13. The method of claim 12 , wherein the receptacle is carried in a receptacle well and the cap is carried in a cap well, and wherein the reagent well, the receptacle well, and the cap well are components of a multi-well tray.

14. The method of claim 5 , wherein the cartridge further comprises an oil reservoir formed in the casing adjacent to the fluid reservoir and comprising a well with an opening at the top surface that is sealed to prevent or limit spillage through the opening, and wherein the opening of the oil reservoir is sealed by a frangible seal secured to the top surface and covering the opening of the oil reservoir, and the method further comprises:

(d) penetrating the frangible seal covering the opening of the oil reservoir with the automated pipettor;

(e) contacting the oil contained in the oil reservoir with the automated pipettor;

(f) withdrawing at least a portion of the oil from the oil reservoir with the automated pipettor;

(g) moving the automated pipettor to the well; and

(h) dispensing at least a portion of the oil withdrawn from the oil reservoir into the well.

15. The method of claim 14 , wherein the frangible seal covering the opening of the oil reservoir comprises a laminate having a foil layer.

16. The method of claim 14 , wherein the laminate further includes a plastic liner.

17. The method of claim 14 ,

wherein the automated pipettor includes a removably attached first pipette tip, and wherein step (a) is performed by puncturing that portion of the frangible seal which covers the opening of the fluid chamber with the first pipette tip and step (c) comprises drawing at least a portion of the liquid into the first pipette tip.

18. The method of claim 17 , further comprising:

dispensing at least a portion of the liquid drawn into the first pipette tip into the well; and

replacing the first pipette tip

with a second pipette tip prior to step (d), wherein step (d) is performed by puncturing that portion of the frangible seal which covers the opening of the oil reservoir with the second pipette tip and step (f) comprises drawing at least a portion of the oil into the second pipette tip.

19. The method of claim 1 , wherein the fluid cartridge further includes a machine-readable identifying label on the casing, and wherein the method further comprises reading information related to the liquid contained in the fluid cartridge from the label.

20. The method of claim 19 , wherein the machine-readable identifying label comprises at least one of a bar code label, a quick response code label, and a radio frequency identification label.

21. The method of claim 10 , further comprising using an automated pipettor to dispense sample material into the receptacle to which the reconstituted reagent is added.

22. The method of claim 21 , wherein the cartridge further comprises an oil reservoir formed in the casing adjacent to the fluid reservoir and comprising a well with an opening at the top surface that is sealed to prevent or limit spillage through the opening, and wherein the opening of the oil reservoir is sealed by a frangible seal secured to the top surface and covering the opening of the oil reservoir, and the method further comprises:

penetrating the frangible seal covering the opening of the oil reservoir with the automated pipettor;

contacting the oil contained in the oil reservoir with the automated pipettor;

withdrawing at least a portion of the oil from the oil reservoir with the automated pipettor;

moving the automated pipettor to the receptacle to which sample material and reconstituted reagent are added; and

dispensing at least a portion of the oil withdrawn from the oil reservoir into the receptacle.