Method for producing butanol using extractive fermentation
View Patent ↗Provided herein are methods for recovering butanol from a fermentation medium. The methods comprise providing a fermentation medium comprising butanol, water, and a recombinant microorganism comprising a butanol biosynthetic pathway, wherein the recombinant microorganism produces butanol; contacting the fermentation medium with a water immiscible organic extractant composition comprising a dry solvent to form a butanol-containing organic phase and an aqueous phase; and recovering the butanol from the butanol-containing organic phase.
1. A method for recovering butanol from a fermentation medium, the method comprising:
a) providing a fermentation medium comprising butanol, water, and a recombinant microorganism comprising a butanol biosynthetic pathway, wherein the recombinant microorganism produces butanol;
b) contacting the fermentation medium with a water immiscible organic extractant composition comprising a dry solvent and a second solvent to form a butanol-containing organic phase and an aqueous phase, wherein the dry solvent is a saturated hydrocarbon that is a branched C7 to C22 alkane or a mixture thereof; and
c) recovering the butanol from the butanol-containing organic phase.
2. The method of claim 1 , wherein the C7 to C22 alkane is a derivative of isobutanol, wherein the derivative of isobutanol is triisobutylene, diisobutylene, tetraisobutylene, isooctane, isohexadecane, or isododecane.
3. The method of claim 2 , wherein the derivative of isobutanol is isododecane.
4. The method of claim 1 , wherein the contacting of the organic extractant composition with the fermentation medium occurs in a fermentor.
5. The method of claim 1 , further comprising transferring a portion of the fermentation medium from a fermentor to a vessel, wherein the contacting of the organic extractant composition with the fermentation medium occurs in the vessel.
6. The method of claim 1 , wherein the second solvent is a C4 to C22 fatty alcohol, a C4 to C28 fatty acid, an ester of a C4 to C28 fatty acid, a C4 to C22 fatty aldehyde, a C7 to C22 ether, a phosphate, an amide, a urea, a phenol, a phosphinate, a carbamate, a phosphoramide, a phosphine oxide, a salicylate, a paraben, or mixtures thereof.
7. The method of claim 6 , wherein the second solvent increases a butanol partition coefficient of the organic extractant composition.
8. The method of claim 1 , wherein the second solvent is oleyl alcohol, behenyl alcohol, cetyl alcohol, lauryl alcohol, myristyl alcohol, stearyl alcohol, oleaic acid, lauric acid, myristic acid, stearic acid, octanoic acid, decanoic acid, undecanoic acid, methyl myristate, methyl oleate, 1-nonanol, 1-decanol, 2-undecanol, 1-nonanal, undecanol, undecanal, isododecanol, 2,6,8-trimethyl-4-nonanol, lauric aldehyde, 2-methylundecanal, oleamide, linoleamide, palmitamide, steaylamide, 2-ethyl-1-hexanol, 2-hexyl-1-decanol, 2-octyl-1-dodecanol, 3,4,5,6,6-pentamethyl-2-heptanol, or mixtures thereof.
9. The method of claim 8 , wherein the second solvent increases a butanol partition coefficient of the organic extractant composition.
10. The method of claim 1 , wherein the recovered butanol has an effective titer from about 30 grams per liter to about 80 grams per liter of the fermentation medium.
11. The method of claim 1 , wherein the recovered butanol has an effective titer of at least 50 grams per liter of the fermentation medium.
12. The method of claim 1 , wherein the butanol is isobutanol.
13. A composition comprising butanol in a water immiscible organic extractant composition, wherein the organic extractant composition comprises a dry solvent and a second solvent, wherein the dry solvent is a saturated hydrocarbon that is a branched C7 to C22 alkane or a mixture thereof.
14. The composition of claim 13 , wherein the C7 to C22 alkane is a derivative of isobutanol, wherein the derivative of isobutanol is triisobutylene, diisobutylene, tetraisobutylene, isooctane, isohexadecane, or isododecane.
15. The composition of claim 13 , wherein the derivative of isobutanol is isododecane.
16. The composition of claim 13 , wherein the second solvent is a C4 to C22 fatty alcohol, a C4 to C28 fatty acid, an ester of a C4 to C28 fatty acid, a C4 to C22 fatty aldehyde, a C7 to C22 ether, a phosphate ester, an amide, a urea, a phenol, a phosphinate, a carbamate, a phosphoramide, a phosphine oxide, a salicylate, a paraben, or mixtures thereof.
17. The composition of claim 13 , wherein the second solvent is oleyl alcohol, behenyl alcohol, cetyl alcohol, lauryl alcohol, myristyl alcohol, stearyl alcohol, oleaic acid, lauric acid, myristic acid, stearic acid, octanoic acid, decanoic acid, undecanoic acid, methyl myristate, methyl oleate, 1-nonanol, 1-decanol, 2-undecanol, 1-nonanal, undecanol, undecanal, isododecanol, 2,6,8-trimethyl-4-nonanol, lauric aldehyde, 2-methylundecanal, oleamide, linoleamide, palmitamide, steaylamide, 2-ethyl-1-hexanol, 2-hexyl-1-decanol, 2-octyl-1-dodecanol, 3,4,5,6,6-pentamethyl-2-heptanol, or mixtures thereof.
18. The composition of claim 13 , wherein the butanol is isobutanol.