Multisignal labeling reagents and processes and uses therefor
Provided are compounds comprising two DNA supramolecular binding molecules covalently joined by a linker group. Also provided are multisignal labeling reagents comprising (i) an oligomer of nucleotides or nucleotide analogs; (ii) a DNA supramolecular binding molecule noncovalently bound to the oligomer; and (iii) a first reactive group or a first partner of a first binding pair covalently bound to the oligomer. Additionally provided are methods of producing multisignal labeling reagents.
1. A multisignal labeling reagent comprising:
(i) an oligomer of nucleotides or nucleotide analogs;
(ii) a plurality of DNA supramolecular binding molecules noncovalently bound to the oligomer,
wherein said DNA supramolecular binding molecules comprise a detectable label attached thereto that is not a DNA supramolecular binding molecule, and
wherein said DNA supramolecular binding molecules are selected from minor groove binders and major groove binders; and
(iii) a first reactive group covalently bound to the oligomer, or
a first partner of a first binding pair covalently bound to the oligomer.
2. The multisignal labeling reagent of claim 1 , wherein the DNA supramolecular binding molecule is fluorescent.
3. The multisignal labeling reagent of claim 1 , wherein the detectable label is non-radioactive.
4. The multisignal labeling reagent of claim 3 , wherein the non-radioactive detectable label is a fluorophore.
5. The multisignal labeling reagent of claim 3 , wherein the non-radioactive detectable label is a chemiluminescent or phosphorescent moiety.
6. The multisignal labeling reagent of claim 1 , wherein the DNA supramolecular binding molecules comprise DNA supramolecular binding molecules which are minor groove binders.
7. The multisignal labeling reagent of claim 6 , wherein the minor groove binders comprise a minor groove binder selected from the group consisting of a diacrylamidine and a bis-benzimidazole.
8. The multisignal labeling reagent of claim 6 , wherein the minor groove binders comprise a minor groove binder selected from the group consisting of DAPI, berenil, pentamidine, distamycin A, and Hoechst 33258.
9. The multisignal labeling reagent of claim 1 , wherein the DNA supramolecular binding molecules comprise DNA supramolecular binding molecules which are major groove binders.
10. The multisignal labeling reagent of claim 9 , wherein the major groove binders comprise a major groove binder selected from the group consisting of a zinc finger, a leucine zipper, and a helix-turn-helix motif.
11. The multisignal labeling reagent of claim 1 , comprising the first partner of the first binding pair covalently bound to the oligomer.
12. The multisignal labeling reagent of claim 11 , wherein the first binding pair is a ligand/receptor, a hormone/receptor, biotin/avidin, biotin/streptavidin or an antigen/antibody binding pair.
13. The multisignal labeling reagent of claim 11 , wherein the first partner of the first binding pair is streptavidin.
14. The multisignal labeling reagent of claim 1 , comprising the first reactive group covalently bound to the oligomer.