Soluble antibody complexes for T cell or NK cell activation and expansion
This disclosure provides compositions and methods for the activation and expansion of human T cells or NK cells using soluble monospecific antibody complexes.
1. A method of activating human T cells comprising culturing a sample containing human T cells with a composition comprising at least one soluble monospecific complex, wherein
(i) each soluble monospecific complex comprises two antibodies or F(ab′) 2 fragments thereof which are linked and bind to the same antigen on the human T cells, and
(ii) the at least one soluble monospecific complex comprises two anti-CD3 antibodies or F(ab′) 2 fragments thereof that bind a CD3 antigen on the human T cells.
2. The method according to claim 1 wherein the composition further comprises a second soluble monospecific complex that comprises two anti-CD28 antibodies or F(ab′) 2 fragments thereof that bind to a CD28 antigen on the human T cells.
3. The method according to claim 2 wherein the composition further comprises a third monospecific antibody complex that comprises two anti-CD2 antibodies that bind a CD2 antigen on the human T cells.
4. The method according to claim 1 wherein the soluble monospecific complexes are tetrameric antibody complexes.
5. The method according to claim 4 wherein the tetrameric antibody complexes (TAC) are composed of two antibodies from one species bound by two antibody molecules from a second species that bind to the Fc portion of the antibodies of the first animal species.