Microfluidic device and method of detecting sample supplied to the same
A microfluidic device includes a chamber configured to accommodate a sample and air, and a detection device provided with a light emitter configured to emit light towards the chamber on a light transmission path and a light receiver configured to receive the light, and further configured to measure an optical density of the chamber to determine whether the sample is accommodated in the chamber based on the received light. A boundary between the sample and the air accommodated in the chamber is provided on the light transmission path.
1. A method of detecting a sample to determine whether an amount of the sample supplied to a microfluidic device is appropriate, the method comprising:
filling a sample distribution chamber with the sample;
moving a remaining portion of the sample, which is remaining after the filling of the sample distribution chamber with the sample, to an excess sample chamber;
moving a part of the remaining portion of the sample moved to the excess sample chamber to a sample accommodating chamber so that a portion of the excess sample chamber is filled with the sample and a remaining portion of the excess sample chamber is filled with air; and
measuring an optical density of the excess sample chamber by using a detection device.
2. The method of claim 1 , wherein the moving of the part of the remaining portion of the sample moved to the excess sample chamber to the sample accommodating chamber comprises:
moving the part of the remaining portion of the sample to the sample accommodating chamber through an excess sample moving channel connected to the excess sample chamber and the sample accommodating chamber.
3. The method of claim 1 , further comprising:
dyeing the sample introduced into the excess sample chamber.
4. The method of claim 1 , wherein in the measuring the optical density of the excess sample chamber, a boundary between the sample and the air in the excess sample chamber is detected.
5. The method of claim 4 , wherein the measuring comprises:
emitting, by a light emitter included in the detection device, light towards the excess sample chamber on a light transmission path;
receiving, by a light receiver included in the detection device, the light; and
measuring the optical density of the excess sample chamber based on the received light.
6. The method of claim 5 , wherein:
the boundary between the remaining portion of the sample and the air accommodated in the excess sample chamber is provided on the light transmission path.
7. The method of claim 5 , wherein:
the excess sample chamber comprises:
a first plate facing the light emitter, and
a second plate facing the light receiver,
wherein the first plate and the second plate comprise one of a hydrophilic material and a hydrophobic material.
8. The method of claim 1 , wherein in the measuring the optical density of the excess sample chamber, an amount of the sample in the excess sample chamber is detected.