Substituted imidazo[1,2-a]pyrazines for use in bioluminogenic methods
View Patent ↗The present invention provides, among other things, imidazo[1,2-α]pyrazine derivatives including The derivatives are useful in any method which other coelenterazines have been used. For example, the derivatives may be used in a bioluminogenic method to detect the presence of certain compounds or molecules.
1. A compound selected from the group consisting of:
2. A kit comprising a compound according to claim 1 .
3. The kit of claim 2 , wherein the kit further comprises a buffer reagent.
4. The kit of claim 2 , wherein the kit further comprises a luciferase.
5. The kit of claim 4 , wherein the luciferase is an Oplophorus or Renilla luciferase.
6. A method for detecting luminescence in a sample, comprising:
contacting the sample with a compound;
contacting the sample with a coelenterazine-utilizing luciferase, if it is not present in the sample; and
detecting luminescence;
wherein the compound is selected from the group consisting of:
7. The method of claim 6 , wherein the sample contains live cells.
8. The method of claim 6 , wherein the sample contains a coelenterazine-utilizing luciferase.
9. A method for detecting luminescence in a transgenic animal expressing a coelenterazine-utilizing luciferase, comprising:
administering to the transgenic animal a compound; and
detecting luminescence;
wherein the compound is selected from the group consisting of: