Detection of indazole synthetic cannabinoids

1. A polyclonal antibody, wherein the polyclonal antibody is raised against an immunogen having the structure of:

wherein KLH is keyhole limpet hemocyanin,

wherein the polyclonal antibody has an IC 50 value of:

1.723 ng/ml to AB-Pinaca N-pentanoic acid;

0.544 ng/ml to 5-fluoropentyl AB-Pinaca;

0.887 ng/ml to 1-(5-hydroxypentyl) AB-Pinaca;

1.107 ng/ml to AB-Pinaca; and

1.546 ng/ml to AB-Fubinaca;

wherein the IC 50 values are determined using a tracer having the structure of:

wherein HRP is horseradish peroxidase and HRP is a detectable label.

2. A kit comprising the polyclonal antibody of claim 1 .

3. A polyclonal antibody, wherein the polyclonal antibody is raised against an immunogen having the structure of:

wherein KLH is keyhole limpet hemocyanin,

wherein the polyclonal antibody exhibits the following percent cross-reactivities for the compounds listed in the table below, when compared with 100% cross reactivity for AB PINACA N-pentanoic acid:

% Cross-

Compound

Reactivity

5-Fluoro AB-PINACA

98.9

5-Hydroxypentyl AB-PINACA

83.8

4-Hydroxypentyl AB-PINACA

85.2

AB-PINACA

52.4

AB-FUBINACA

35.3

ADB-PINACA pentanoic acid metabolite

32.8

5-Fluoro AB-PINACA N-(4-hydroxypentyl)

24.1

metabolite

ADB-PINACA N-(5-hydroxypentyl) metabolite

15.2

5-Fluoro ADB-PINACA

9.8

5-Fluoro ADBICA

4.7

AB-FUBINACA carboxylic acid

4.5

AB-CHMINACA

3.8

ADBICA

0.7

AB-PINACA carboxylic acid

<1

AKB48 N-(4-hydroxypentyl) metabolite

<1

AKB48 N-5-hydroxypentyl) metabolite

<1

PB-22 N-(5-hydroxypentyl) metabolite

<1

MN-25

<1

PB-22 N-Pentanoic acid

<1

AKB48 N-Pentanoic acid metabolite

<1

MN-18

<1

RCS-4

<1

3-OH AKB-48

<1

SDB-001 N-Pentanoic acid

<1

3-OH SDB-001

<1

AB-001 N-Pentanol

<1

wherein the cross-reactivities are determined by using a tracer having the structure of:

wherein HRP is horseradish peroxidase and HRP is a detectable label.

4. A kit comprising the polyclonal antibody of claim 3 .

5. A method of detecting or determining one or more compounds selected from the group consisting of AB-Pinaca, AB-Pinaca pentanoic acid, 1-(5-hydroxypentyl) AB-Pinaca, 1-(4-hydroxypentyl) AB-Pinaca, and 5-fluoropentyl AB-Pinaca, in an in vitro sample or in a solution comprising:

i. contacting the sample or solution with a detecting agent and the polyclonal antibody of claim 1 , wherein both the compound and the detecting agent bind to the polyclonal antibody; and

ii. detecting or determining the amount of detecting agent bound to the polyclonal antibody.

6. The method of claim 5 , wherein the polyclonal antibody is adsorbed on or attached to a solid state device.

7. The method of claim 6 , wherein the solid state device is a ceramic biochip or microtiter plate.

8. A method of making an antibody comprising:

administering an immunogen having the structure of:

wherein “Carrier” is selected from the group consisting of Keyhole Limpet Hemocyanin (KLH) and Bovine Serum Albumin (BSA),

to a mammal; and

purifying the resultant antibody.

9. The method of claim 8 , wherein the antibody is a polyclonal antibody.