Glucosyltransferase enzymes
A process for producing poly alpha-1,3-glucan with reduced molecular weight is disclosed. The process comprises contacting water, sucrose, a polar organic solvent, and a glucosyltransferase enzyme in a solution to produce poly alpha-1,3-glucan. This contacting step results in the production of poly alpha-1,3-glucan having a reduced molecular weight compared to the molecular weight of a poly alpha-1,3-glucan made in the absence of the polar organic solvent.
1. An isolated protein comprising SEQ ID NO: 14, wherein said protein has glucosyltransferase activity and produces poly alpha-1,3-glucan having at least 50% alpha-1,3 glycosidic linkages.
2. An isolated protein consisting of SEQ ID NO: 14.
3. An isolated protein that is a fragment of the polypeptide of SEQ ID NO: 32, wherein said fragment consists of an amino acid sequence that is at least 95% identical with SEQ ID NO: 14, and wherein said protein has glucosyltransferase activity and produces poly alpha-1,3-glucan having at least 50% alpha-1,3 glycosidic linkages.
4. The protein of claim 3 , wherein the fragment consists of an amino acid sequence that is at least 97% identical with SEQ ID NO:14.
5. The protein of claim 4 , wherein the fragment consists of an amino acid sequence that is at least 98% identical with SEQ ID NO:14.
6. The protein of claim 3 , wherein said poly alpha-1,3-glucan has at least 95% alpha-1,3 glycosidic linkages.
7. A reaction solution comprising water, sucrose and a protein comprising SEQ ID NO: 14, wherein said protein has glucosyltransferase activity and produces poly alpha-1,3-glucan having at least 50% alpha-1,3 glycosidic linkages.
8. A reaction solution comprising water, sucrose and a protein consisting of SEQ ID NO: 14.
9. A reaction solution comprising water, sucrose and a protein that is a fragment of the polypeptide of SEQ ID NO: 32, wherein said fragment consists of an amino acid sequence that is at least 95% identical with SEQ ID NO: 14, and wherein said protein has glucosyltransferase activity and produces poly alpha-1,3-glucan having at least 50% alpha-1,3 glycosidic linkages.
10. The reaction solution of claim 9 , wherein the fragment consists of an amino acid sequence that is at least 97% identical with SEQ ID NO:14.
11. The reaction solution of claim 10 , wherein the fragment consists of an amino acid sequence that is at least 98% identical with SEQ ID NO:14.
12. The reaction solution of claim 9 , wherein said poly alpha-1,3-glucan has at least 95% alpha-1,3 glycosidic linkages.