IP Library › Granted Patent US 10,138,464
Granted Patent B2
US 10,138,464 · App. 14/863,274 · Granted Nov 27, 2018

Use of apoptotic cells ex vivo to generate regulatory T cells

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Quick Facts
Patent No.
US 10,138,464
App. No.
14/863,274
Granted
Nov 27, 2018
Kind
B2
Abstract

Many cell types in the body can remove apoptotic and cellular debris from tissues; however, the professional phagocyte, or antigen presenting cell (“APC”), has a high capacity to do so. The recognition of apoptotic cells (“ACs”) occurs via a series of evolutionarily-conserved, AC associated molecular-pattern receptors (“ACAMPRs”) on APCs that recognize and bind corresponding apoptotic-cell-associated molecular patterns (“ACAMPs”). These receptors recognize ligands such as phosphotidyl serine and oxidized lipids found on apoptotic cells. Savill et al. (2002); and Gregory et al. (2004).

Claims (11)

1. A method of generating T cells with regulatory activity (T regs), the method comprising incubating CD4+ leukocytes with autologous apoptotic peripheral blood mononuclear cells (ACs) and IL-10.

2. The method according to claim 1 , wherein the incubation is at about a 1:10 to about a 10:1 ratio CD4+:ACs.

3. The method according to claim 2 wherein the incubation is at about a 2:1 to about a 1:2 ratio CD4+:ACs.

4. The method according to claim 1 wherein the ACs are obtained by an apoptosis-inducing treatment.

5. The method according to claim 4 wherein the apoptosis-inducing treatment is an ECP procedure that employs a photoactivatable compound together with light of a wavelength that activates the photoactivable compound.

6. The method according to claim 5 wherein the photoactivable compound is a psoralen and the light is UVA.

7. The method according to claim 6 wherein the psoralen is 8-MOP.

8. The method according to claim 1 , wherein the IL-10 is present at a concentration of about 1 ng/ml to about 100 ng/ml.

9. The method according to claim 8 wherein the IL-10 is present at a concentration of about 20 ng/ml.

10. The method according to claim 1 wherein the incubation is for about 1 to about 14 days.

11. The method according to claim 10 wherein the incubation is for about 8 days.

Assignments (19)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 10, 2026
From: MALLINCKRODT HOSPITAL PRODUCTS IP UNLIMITED COMPANY
To: MALLINCKRODT PHARMACEUTICALS IRELAND LIMITED COMPANY
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ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 23, 2026
From: THERAKOS DEVELOPMENT LIMITED
To: THERAKOS HOLDINGS USA, LLC
Reel/Frame 075227/0886 →
RELEASE OF PATENT SECURITY INTEREST RECORDED AT REEL 69476, FRAME 0380 Recorded Mar 17, 2026
From: NOMURA CORPORATE FUNDING AMERICAS, LLC, AS ADMINISTRATIVE AGENT AND COLLATERAL AGENT
To: THERAKOS DEVELOPMENT LIMITED
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PATENT SECURITY AGREEMENT Recorded Mar 17, 2026
From: THERAKOS HOLDINGS USA, LLC
To: NOMURA CORPORATE FUNDING AMERICAS, LLC, AS ADMINISTRATIVE AGENT AND COLLATERAL AGENT
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PATENT SECURITY AGREEMENT Recorded Dec 3, 2024
From: SOLARIS IPCO LIMITED
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RELEASE OF PATENT SECURITY INTERESTS RECORDED AT REEL 043601, FRAME 0025 Recorded Nov 16, 2023
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RELEASE OF PATENT SECURITY INTERESTS RECORDED AT REEL 060434, FRAME 0536 Recorded Nov 16, 2023
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SECURITY INTEREST Recorded Jun 17, 2022
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