Apparatuses containing an emulsion and methods of manufacture
A method for preparing a card containing at least one sequestered compound is provided. The method includes preparing a first waxy phase, preparing a first volatile phase comprising a first sequestered compound, creating a first emulsion from the first waxy phase and the first volatile phase, depositing the first emulsion on the card, and cooling the first emulsion.
1. A method for preparing a card for use with an experimental animal, the card containing at least one sequestered volatile compound, the method comprising:
preparing a first solid phase, the first solid phase comprising one or more of Octadecane, Nonadecane, Icosane, Heneicosane, Docosane, Tricosane, and/or Tetracosane;
preparing a first volatile phase comprising a first volatile compound, the first volatile compound comprising (i) a carrier comprising a low-molecular-weight hydrocarbon or alcohol and (ii) an odiferous compound that is detectable as an olfactory stimulus by the experimental animal or a drug;
creating a first emulsion of the first volatile phase dispersed inside the first solid phase;
depositing the first emulsion on the card; and
cooling the first emulsion.
2. The method of claim 1 , wherein creating the first emulsion comprises vigorously mixing the first solid phase and the first volatile phase into a colloidal suspension such that the first volatile phase forms microvesicles within the first solid phase.
3. The method of claim 1 , wherein creating the first emulsion comprises using a sonicator or nebulizer to create the first emulsion.
4. The method of claim 1 , wherein preparing the first volatile phase comprises dissolving the first volatile compound into the carrier solution.
5. The method of claim 1 , wherein depositing the first emulsion comprises depositing the first emulsion as a series of first droplets.
6. The method of claim 1 , wherein the first solid phase has a melting point between 28° C. and 54° C.
7. The method of claim 1 , wherein the first volatile compound comprises a drug, the drug comprising a trace amine type hormone.
8. The method of claim 1 , further comprising: providing one or more heat-receiving elements on the card, wherein the first emulsion is deposited onto the card opposite from the one or more heat-receiving elements or is deposited directly onto the one or more heat-receiving elements.
9. The method of claim 1 , further comprising: providing one or more metallic heat-receiving elements on the card.