Fecal Recovery Assay
The fecal recovery assay of this invention accurately quantifies the number of viable BB12 colonies in the gastrointestinal tract of humans and animals. This BB12 culture method is highly selective for isolating BB12 colonies from human stool. Furthermore, this method has the utility of assessing the survivability of BB12 delivered by food products and supplements through the GI tract, and thus providing potential information on the human health effects of viable BB12.
1 . A method for measuring recovery or survival of probiotic organisms in human fecal samples from the gastrointestinal tract of a subject.
1 . The method of claim 1 wherein the probiotic organisms in the human fecal samples are isolated and enumerated.
2 . The method of claim 1 wherein the probiotic organisms that are isolated and enumerated are selected from Bifidobacterium animalis , subspecies lactis BB12 and Lactobacillus rhamnosus GG.
3 . The method of claim 1 wherein the Bifidobacterium animalis , subspecies lactis BB12 and Lactobacillus rhamnosus GG are enumerated using quantitative culture confirmed by PCR.
4 . The method of claim 1 wherein the Bifidobacterium animalis , subspecies lactis BB12 and Lactobacillus rhamnosus GG are enumerated using quantitative PCR.
5 . The method of claim 1 wherein the Bifidobacterium animalis , subspecies lactis BB12 is isolated in fecal samples by culture on MRS agar with added tetracycline.