Expression cassettes derived from maize
View Patent ↗The present invention includes expression cassettes that contain regulatory sequences derived from a target gene, for example, regulatory sequences from the HSP70, Ubi158, and Ubi361 genes, for expression of recombinant gene products in plants. Developmental expression profiling data were used to identify several gene candidates for strong constitutive expression cassette development. Three expression cassettes were developed. They are based on the ZmHSP70, ZmUbi158, and ZmUBI361 genes.
1. An expression cassette comprising a promoter operably linked to a heterologous gene, wherein the promoter is capable of driving constitutive expression in a plant cell and comprises a nucleic acid sequence that is at least 99% identical to SEQ ID NO: 3.
2. A method of constitutively expressing a heterologous gene comprising:
a. constructing an expression cassette comprising a promoter comprising a nucleic acid sequence that is at least 99% identical to SEQ ID NO: 3, operably linked to a heterologous gene, wherein the expression cassette is functional in a plant, plant cell, or plant tissue; and
b. creating a plant, plant cell, or plant tissue or portion thereof comprising the expression cassette,
wherein the heterologous gene is expressed.
3. The method of claim 2 , wherein the plant, plant cell, or plant tissue or portion thereof is a monocot.
4. The method of claim 3 , wherein the plant, plant cell, or plant tissue or portion thereof is maize.
5. A plant, plant cell, or plant tissue or portion thereof comprising the expression cassette of claim 1 .
6. The plant, plant cell, or plant tissue or portion thereof of claim 5 , wherein the plant, plant cell, or plant tissue is a monocot.
7. The plant, plant cell, or plant tissue or portion thereof of claim 5 , wherein the plant, plant cell, or plant tissue is maize.
8. The plant, plant cell, or plant tissue or portion thereof of claim 5 , wherein the plant, plant cell, or plant tissue is a dicot.
9. The plant, plant cell, or plant tissue or portion thereof of claim 5 , wherein the plant, plant cell, or plant tissue is tobacco or soybean.
10. A plant, plant cell, or plant tissue or portion thereof made by the method of claim 2 , wherein the plant, plant cell, or plant tissue or portion thereof comprises an expression cassette comprising a promoter comprising a nucleic acid sequence that is at least 99% identical to SEQ ID NO: 3, operably linked to a heterologous gene, wherein the expression cassette is functional in said plant, plant cell, or plant tissue.
11. A progeny of the plant, plant cell, or plant tissue or portion thereof, of claim 10 , wherein the progeny of the plant, plant cell, or plant tissue or portion thereof comprises the expression cassette comprising a promoter comprising a nucleic acid sequence that is at least 99% identical to SEQ ID NO: 3, operably linked to a heterologous gene, wherein the expression cassette is functional in said plant, plant cell, or plant tissue.
12. A seed derived from the progeny of claim 11 , wherein the seed comprises the expression cassette of claim 11 .
13. A grain from the seed of claim 12 , wherein the grain comprises the expression cassette of claim 11 .