Customized repair constructs and method of preparation
Customized, three-dimensionally printed constructs useful for promoting tissue repair and methods of preparing the same are provided.
1. A method for preparing a customized tissue repair construct for a specific tissue repair site comprising
measuring the tissue repair site via a three-dimensional scanner or computed tomography (CT) scanner;
preparing a scaffold according to an additive manufacturing process selected from the group consisting of direct metal laser sintering (DMLS) process, an electron beam melting (EBM) process, selective laser sintering (SLS), stereolithography (SLA), and laminated object manufacturing; and
infiltrating the scaffold with a flowable/injectable human birth tissue product consisting of amniotic fluid and human amniotic membrane to form a tissue repair construct,
wherein the scaffold consists of one or more biomaterials selected from the group consisting of human placenta derived extracellular matrix (ECM), glycosaminoglycans, fibronectin, and laminin, and
wherein the scaffold is prepared according to a predetermined size based on the size of the tissue repair site thereby resulting in a customized tissue repair construct.
2. A method of tissue repair comprising
measuring a tissue repair site;
preparing a scaffold according to a bioprinting process;
infiltrating the scaffold with a flowable/injectable human birth tissue product consisting of amniotic fluid and human amniotic membrane to form a tissue repair construct; and
applying the tissue repair construct to the tissue repair site,
wherein the tissue repair construct aids in healing the tissue repair site,
wherein the scaffold consists of one or more biomaterials selected from the group consisting of human placenta derived extracellular matrix (ECM), glycosaminoglycans, fibronectin, and laminin, and
wherein the step of measuring the tissue repair site is performed by a three-dimensional scanner or computed tomography (CT) scanner.