Mixing and filtering system and method

1. A mixing container assembly which can be used to grow and separate cells or micro carriers from liquid or fluid medium in an aseptic environment, comprising:

a container having a sealing mechanism for being sealed both air and liquid tight, wherein the container has a bottom;

at least one port through a portion of the container to allow for the introduction of fluid, gas or components;

a separation unit having a filter with a filtration medium mounted centrally around an axis extending through the container;

a mixing assembly mounted for rotation around an outer periphery of the filtration medium to allow for suspension of media or agitation thereof,

wherein the mixing assembly and filter have bottom portions that are proximate the bottom of the container;

the mixing assembly has vanes containing a magnetic unit in a lower portion thereof proximate the bottom of the container that allows for coupling to a stir plate for driving the vanes of the mixing assembly from below the bottom of the container;

a central support structure which serves as an axis of rotation for the vanes and as a support for the separation unit;

an integral bearing device mounted around the central support structure that allows the mixing assembly to rotate around the mounted filtration medium while the mounted filtration medium is stationary;

a central diptube extending through the filter;

wherein the mixing assembly is suspended from above the separation unit by the integral bearing device; and

wherein the vanes that extend proximate the bottom of the container are supported by the bearing device from above the separation unit, wherein the bearing device is remote from the bottom of the container with respect to the separation unit and remote from the magnetic unit with respect to the vanes, and wherein the separation unit, the vanes, and the central diptube all extend downward in the container to substantially the same clearance from the bottom of the container.

2. The mixing container assembly of claim 1 , wherein the container has a flat bottom.

3. The mixing container assembly of claim 1 , wherein the central diptube extends from the top of the container proximate the bottom of the container.

4. The mixing container assembly of claim 1 , wherein the magnetic unit comprises a split magnet in which there are two magnets oriented in opposing directions and spaced from each other more than a diameter of the separation unit, whereby the separation unit is disposed between the magnets.

5. The mixing container assembly of claim 1 , wherein the container is a rigid container.

6. The mixing container assembly of claim 1 , wherein the bearing device is located inside a bore in a top portion of the mixing assembly.

7. The mixing container assembly of claim 1 , wherein the bottom of the mixing assembly extends laterally away from the bottom of the filter and adjacent to an interior bottom of the container to help agitate particles that would otherwise rest at the bottom of the container.

8. The mixing container assembly of claim 1 , wherein the top of the mixing assembly is narrower than the bottom of the mixing assembly.

9. The mixing container assembly of claim 1 , wherein the magnetic unit comprises magnets in a bottom portion of at least two vanes.

10. The mixing container assembly of claim 1 , wherein the magnetic unit comprises two magnets that are the same size and shape at a low point of the mixing vanes of the mixing assembly, with one magnet being located pointing its north pole in the downward position and the other is pointing its opposing south pole in the downward position.