Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
View Patent ↗The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
1. An engineered polynucleotide encoding an engineered polypeptide having imine reductase activity, wherein said polynucleotide encodes the polypeptide of SEQ ID NO: 96.
2. The engineered polynucleotide of claim 1 , wherein said polypeptide is capable of converting substrate compound (1a) pyruvate,
and substrate compound (2b) butylamine
to product compound (3b) N-2-(butylamino)propanoic acid
under suitable reaction conditions.
3. The engineered polynucleotide of claim 1 , wherein said polypeptide is capable of converting substrate compound (1b) cyclohexanone,
and substrate compound (2a) L-norvaline
to product compound (3c) (S)-2-(cyclohexylamino)pentanoic acid,
under suitable reaction conditions.
4. The engineered polynucleotide of claim 1 , wherein said polypeptide is capable of converting substrate compound (1b) cyclohexanone,
and substrate compound (2b) butylamine
to product compound (3d) N-butylcyclohexanamine,
under suitable reaction conditions.
5. The engineered polynucleotide of claim 1 , wherein said polypeptide is capable of converting substrate compound (1i),
and substrate compound (2b)
to product compound (3n)
under suitable reaction conditions.
6. The engineered polynucleotide of claim 1 , wherein said polypeptide is capable of converting substrate compound (1j),
and substrate compound (2b)
to product compound (3o)
under suitable reaction conditions.