IP Library Granted Patent US 9,373,486
Granted Patent B2
US 9,373,486 · App. 14/889,137 · Granted Jun 21, 2016

Species detection using mass spectrometry

Inventor: Stephen Lock (West Yorkshire, GB)
Assignee: DH Technologies Development Pte. Ltd.
H01J49/0036G01N33/12H01J49/004
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Quick Facts
Patent No.
US 9,373,486
App. No.
14/889,137
Granted
Jun 21, 2016
Kind
B2
Abstract

Systems and methods are provided for species detection using mass spectrometry. In various embodiments, a multiple reaction monitoring (MRM) experiment is performed on a sample targeting one or more peptide transitions that are unique to one or more species using a tandem mass spectrometer. One or more measured product ion spectra are received from the tandem mass spectrometer using the processor. The one or more measured product ion spectra are compared to product ions of the one or more peptide transitions that are unique to one or more species using the processor. One or more species of the sample are detected by reporting product ions of the one or more peptide transitions that are unique to one or more species that match the one or more measured product ion spectra using the processor.

Claims (40)

1. A system for species detection using tandem mass spectrometry, comprising:

a tandem mass spectrometer that performs a multiple reaction monitoring (MRM) experiment on a sample targeting one or more peptide transitions that are unique to one or more species; and

a processor in communication with the tandem mass spectrometer that

receives one or more measured product ion spectra from the tandem mass spectrometer,

compares the one or more measured product ion spectra to product ions of the one or more peptide transitions that are unique to one or more species, and

detects one or more species of the sample by reporting product ions of the one or more peptide transitions that are unique to one or more species that match the one or more measured product ion spectra.

2. The system of claim 1 , further comprising a separation device that separates the sample from a mixture.

3. The system of claim 1 , wherein the sample is prepared using a solid phase extraction (SPE).

4. The system of claim 1 , wherein the sample comprises meat.

5. The system of claim 1 , wherein the sample comprises processed meat.

6. The system of claim 1 , wherein the sample is prepared without using a size exclusion technique.

7. The system of claim 1 , wherein the sample is prepared without using deoxyribonucleic acid (DNA) amplification.

8. The system of claim 1 , wherein the tandem mass spectrometer performs an enhanced product ion (EPI) scan during the MRM experiment.

9. The system of claim 1 , wherein

the tandem mass spectrometer further targets one or more transitions of one or more known small molecules in the same MRM experiment,

the processor further

compares the one or more measured product ion spectra to small molecule product ions of the one or more transitions of one or more known small molecules, and

detects one or more known small molecules of the sample along with the one or more species of the sample by reporting small molecule product ions of the one or more transitions of one or more known small molecules that match the one or more measured product ion spectra.

10. The system of claim 9 , wherein the one or more known small molecules comprise residues or metabolites of one or more of veterinary drugs, phenylbutazone (BUTE), antibiotics, growth hormones, and pesticides.

11. A method for species detection using tandem mass spectrometry, comprising:

performing a multiple reaction monitoring (MRM) experiment on a sample targeting one or more peptide transitions that are unique to one or more species using a tandem mass spectrometer;

receiving one or more measured product ion spectra from the tandem mass spectrometer using a processor;

comparing the one or more measured product ion spectra to product ions of the one or more peptide transitions that are unique to one or more species using the processor; and

detecting one or more species of the sample by reporting product ions of the one or more peptide transitions that are unique to one or more species that match the one or more measured product ion spectra using the processor.

12. The method of claim 11 , wherein the sample comprises meat.

13. The method of claim 11 , further comprising

targeting one or more transitions of one or more known small molecules in the same MRM experiment using the tandem mass spectrometer,

comparing the one or more measured product ion spectra to small molecule product ions of the one or more transitions of one or more known small molecules using the processor, and

detecting one or more known small molecules of the sample along with the one or more species of the sample by reporting small molecule product ions of the one or more transitions of one or more known small molecules that match the one or more measured product ion spectra using the processor.

14. The method of claim 13 , wherein the one or more known small molecules comprise residues or metabolites of one or more of veterinary drugs, phenylbutazone (BUTE), antibiotics, growth hormones, and pesticides.

15. The method of claim 11 , wherein the sample is prepared using a solid phase extraction (SPE).

16. The method of claim 11 , wherein the sample comprises processed meat.

17. The method of claim 11 , wherein the sample is prepared without using a size exclusion technique.

18. The method of claim 11 , wherein the sample is prepared without using deoxyribonucleic acid (DNA) amplification.

19. The method of claim 11 , wherein the tandem mass spectrometer performs an enhanced product ion (EPI) scan during the MRM experiment.

20. A computer program product, comprising a tangible computer-readable storage medium whose contents include a program with instructions being executed on a processor so as to perform a method for species detection using mass spectrometry, the method comprising:

providing a system, wherein the system comprises one or more distinct software modules, and wherein the distinct software modules comprise a measurement module and a detection module;

receiving one or more measured product ion spectra from a tandem mass spectrometer that performs a multiple reaction monitoring (MRM) experiment on a sample targeting one or more peptide transitions that are unique to one or more species using the measurement module;

comparing the one or more measured product ion spectra to product ions of the one or more peptide transitions that are unique to one or more species using the detection module; and

detecting one or more species of the sample by reporting product ions of the one or more peptide transitions that are unique to one or more species that match the one or more measured product ion spectra using the detection module.

Assignments (2)
CHANGE OF ADDRESS Recorded May 6, 2016
From: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
To: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Reel/Frame 038631/0857 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 5, 2015
From: LOCK, STEPHEN
To: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
Reel/Frame 036967/0071 →
Continuity (2)
Provisional Application 61825935 · May 21, 2013
Related Publication 20160086782A1 · Mar 24, 2016