Processes for the preparation of dehydroepiandrosterone and its intermediates
The present application relates to a regioselective and stereoselective processes for the preparation of dehydroepi-androsterone (DHEA) and processes for its intermediates.
1. A process for preparing 3β-hydroxyandrost-5-en-17-one of formula (I)
comprising reducing 3-oxo group of Δ 5 -androstene-3,17-dione of formula (II)
using a ketoreductase enzyme having SEQ ID NO:1.
2. A process for preparing the compound of formula (III)
comprising:
acetylating the compound of formula (I), wherein the compound of formula (I) is prepared according to claim 1 ,
3. A process for the preparing compound of formula (III)
comprising the steps of:
a) isomerizing Δ 4 -androstene-3,17-dione of formula (IV)
to give Δ 5 -androstene-3,17-dione of formula (II);
b) regioselectively and stereoselectively reducing the 3-oxo group of Δ 5 -androstene-3,17-dione of formula (II) using a ketoreductase enzyme, wherein the compound of formula (II) is prepared according to claim 1 to obtain 3β-hydroxyandrost-5-en-17-one of formula (I); and
c) acetvlatinq the compound of formula (I) to obtain the compound of formula (III).
4. The process of claim 1 , wherein compound of formula (I) is converted to Abiraterone acetate.
5. The process of claim 1 , wherein the compound of formula (I) is converted to DHEA Enanthate.