Markers to predict macrocyclic lactone drug resistance in dirofilaria immitis, the causative agent of heartworm disease
View Patent ↗Disclosed are nucleic acid molecules from the genome of Dirofilaria spp. nematodes that contain single nucleotide polymorphisms related to reduced responsiveness of the nematodes to macrocyclic lactones. In one example, the species of Dirofilaria is Dirofilaria immitis (the agent of heartworm in animals). Also disclosed are methods for determining the responsiveness of Dirofilaria spp. nematodes to macrocyclic lactones, methods for selecting a treatment to treat an animal infected with a Dirofilaria spp. nematode, and kits for determining the responsiveness of Dirofilaria spp. nematodes to macrocyclic lactones.
1. A method for determining the genotype of a Dirofilaria immitis nematode, the method comprising:
a) contacting a Dirofilaria immitis nucleic acid molecule with at least one oligonucleotide having a length of 15-300 nucleotides of SEQ ID NO: 118, or a reverse complement thereof;
wherein the at least one oligonucleotide comprises a G nucleotide at position 151 of SEQ ID NO: 118;
wherein the at least one oligonucleotide further includes a detectable label; and
b) detecting a G nucleotide at position 151 of SEQ ID NO: 118 in the Dirofilaria immitis nematode nucleic acid molecule.
2. The method of claim 1 , wherein the at least one oligonucleotide has a length of 15-100 nucleotides.
3. The method of claim 1 , wherein the at least one oligonucleotide has a length of 20-30 nucleotides.
4. The method of claim 1 , wherein the detection of the G nucleotide at position 151 of SEQ ID NO: 118 in the Dirofilaria immitis nucleic acid molecule with the at least one oligonucleotide is detected by DNA sequencing, hybridization-based methods including with allele specific oligonucleotides, microarray analysis, enzyme-based methods, single strand conformational polymorphism (SSCP), high resolution melt (HRM) or approaches based on PCR, RT-PCR, or qRT-PCR.
5. The method of claim 1 , further comprising:
a) contacting the Dirofilaria immitis nucleic acid molecule with at least a second oligonucleotide consisting of 15-300 nucleotides of a sequence selected from the group consisting of SEQ ID NOs: 1-113, 115-117, and 119-127, or a reverse complement thereof;
wherein the at least a second oligonucleotide comprises position 151 of the sequence selected from the group consisting of SEQ ID NOs: 1-113, 115-117, and 119-127;
wherein the at least a second oligonucleotide further comprises a detectable label, and
b) detecting the nucleotide at position 151 of the sequence selected from the group consisting of SEQ ID NOs: 1-113, 115-117, and 119-127 in the Dirofilaria immitis nematode nucleic acid molecule.