Compositions and methods for clostridial transformation
The invention provides compositions and methods for clostridial bacteria that have been engineered to produce and/or to improve efficiency of production of industrial bioproducts.
1. A method of producing a recombinant Clostridium bacterial transformant, comprising:
a) introducing a polynucleotide encoding for a DNA methyltransferase into a Escherichia bacterial host cell, wherein the polynucleotide encoding for a DNA methyltransferase has at least 90% sequence identity to SEQ ID NO: 1,
b) culturing the Escherichia bacterial host cell under conditions suitable for expression of the DNA methyltransferase, wherein the encoded DNA methyltransferase methylates a polynucleotide,
c) transferring the methylated polynucleotide from the Escherichia bacterial host cell to a Clostridium bacterial host cell, wherein the bacteria transformed using this method are selected from the group consisting of Clostridium aceticum, Clostridium ljungdahlii, Clostridium acetobutylicum , and Clostridium autoethanogenum.
2. The method of claim 1 , wherein the polynucleotide encoding for a DNA methyltransferase is SEQ ID NO: 2.
3. The method of claim 1 , wherein the encoded DNA methyltransferase methylates a polynucleotide at a sequence comprising CCWGG.
4. The method of claim 3 , wherein the sequence comprising CCWGG is selected from the group consisting of CCAGG (SEQ ID NO: 9) and CCTGG (SEQ ID NO: 10).