Pet imaging of zinc transport
A method for producing a solution including a positron emitting zinc cation of 63 Zn, and the method comprises bombarding a solution target including 63 Cu with high energy protons to produce a solution including a positron emitting zinc cation. A method for detecting or ruling out Alzheimer's disease in a patient comprises administering to a patient a detectable amount of a compound including a positron emitting zinc cation, wherein the zinc cation is targeted to beta-amyloid in the patient, and acquiring an image to detect the presence or absence of beta-amyloid in the patient.
1. A method for producing a solution including a positron emitting zinc cation, the method comprising:
bombarding a salt solution target including copper cations of 63 Cu with high energy protons to produce a solution including a positron emitting 63 Zn cation wherein the salt solution target is a nitrate salt solution.
2. The method of claim 1 , wherein the salt solution target is produced by dissolving a 63 Cu powder in an acid to form a 63 Cu salt solution.
3. The method of claim 1 , wherein the salt solution target is produced by dissolving a 63 Cu powder in an acid to form a 63 Cu salt solution; and diluting the 63 Cu salt solution to form the solution target.
4. The method of claim 2 , wherein the 63 Cu powder comprises at least 99% of 63 Cu.
5. The method of claim 2 , wherein the acid is nitric acid.
6. The method of claim 1 , further comprising:
purifying the solution of the positron emitting zinc cation to remove impurities.
7. The method of claim 6 , wherein the impurities comprise 11 C and 13 N.
8. The method of claim 1 , wherein the method further comprises:
passing the solution including the positron emitting zinc cation through a column including a sorbent to adsorb the positron emitting zinc cation on the sorbent; and
eluting the positron emitting zinc cation off the sorbent.
9. The method of claim 8 , wherein the elution medium is 0.05 N HCl in 90% acetone.
10. The method of claim 1 , wherein the method further comprises:
passing the solution including the positron emitting zinc cation through a column including a sorbent to adsorb the positron emitting zinc cation on the sorbent; and
eluting the positron emitting zinc cation off the sorbent to create a positron emitting zinc chloride solution.
11. The method of claim 10 , wherein the method further comprises:
purifying the positron emitting zinc chloride solution by neutralizing the solution with a basic solution.
12. The method of claim 11 , wherein the method further comprises:
trapping positron emitting zinc on a column comprising carboxymethyl derivatized resin;
washing the resin with a dilute basic solution; and
eluting the positron emitting zinc from the column by passing a 4% sodium citrate solution through the resin.