Fluidic cartridge for nucleic acid amplification and detection

1. A cartridge for assay of a target nucleic acid sequence in a liquid sample comprising a plastic housing enclosing:

a. a plastic fluidic portion comprising a channel fluidly connecting a sample inlet to at least one amplification chamber where nucleic acid amplification can take place; a channel fluidly connecting the amplification chamber to at least one detection chamber where the results of nucleic acid amplification can be electrochemically detected; an exhaust; and a gas inlet connectable to a gas supply which is capable of pushing the sample downstream through the cartridge,

wherein the channels and/or chambers comprise at least one pneumatically controlled valve,

and wherein upstream of the amplification chamber are provided: reagents and/or physical components for cell lysis and nucleic acid separation; in or upstream of the amplification chamber, but downstream of the reagents and/or physical components for cell lysis and nucleic acid separation, are provided reagents for nucleic acid amplification including a nucleic acid polymerase; and in or upstream of the detection chamber, but downstream of the amplification chamber, are provided reagents for electrochemical nucleic acid detection;

b. a plastic pneumatic portion comprising at least one pneumatic pressure inlet and pneumatic circuit for conveying pneumatic pressure;

whereby flow through the channels and chambers can be controlled by the pneumatic pressure applied to the at least one pneumatically controlled valve; and

c. at least two electrodes capable of providing a potential difference across the at least one detection chamber for permitting detection of electrochemically active molecules.

2. The cartridge of claim 1 , wherein the fluidic portion further comprises a control nucleic acid.

3. The cartridge of claim 2 , wherein the control nucleic acid is DNA.

4. The cartridge of claim 1 , wherein the target nucleic acid is DNA.

5. The cartridge of claim 4 , wherein the target DNA is from a pathogen.

6. The cartridge of claim 1 , wherein the reagents for nucleic acid amplification further comprise primers capable of hybridizing to the target nucleic acid sequence, dNTPs, and optionally further comprises one or more of buffer salts, MgCl2, passivation agents, uracil N-glycosylase and dUTP.

7. The cartridge of claim 2 , wherein the reagents for nucleic acid amplification further comprise primers capable of hybridizing to the control nucleic acid.

8. The cartridge of claim 1 , wherein the reagents for electrochemical nucleic acid detection comprise at least one target probe comprising a label, wherein the target probe is capable of specifically hybridizing to the target molecule.

9. The cartridge of claim 2 , wherein the reagents for electrochemical nucleic acid detection comprise at least one control probe comprising a label, wherein the control probe is capable of specifically hybridizing to the control molecule.

10. The cartridge of claim 8 , wherein the label is a ferrocene label.

11. The cartridge of claim 1 , wherein the reagents for nucleic acid detection comprise a 5′ to 3′ exonuclease.

12. The cartridge of claim 1 , wherein the pneumatic portion comprises multiple pneumatic pressure inlets each of which controls flow through separate regions of the fluidic portion.

13. The cartridge of claim 1 , wherein flow through the channels and chambers is controlled by multiple pneumatically controlled valves.

14. The cartridge of claim 1 , wherein the fluidic portion further comprises a coarse filter downstream of the sample inlet and upstream of the reagents and/or physical components for cell lysis and nucleic acid separation.

15. The cartridge of claim 1 , wherein the reagents and/or physical components for cell lysis and nucleic acid separation include lysis buffer.

16. The cartridge of claim 1 , wherein the reagents and/or physical components for cell lysis and nucleic acid separation include a nucleic acid capture column.

17. The cartridge of claim 1 , wherein the fluidic portion further comprises one or more of a sample mixing chamber, a sample indicator, a bellows, a mixing channel, a blister sub-assembly, an isolation valve, an elution chamber.

18. The cartridge of claim 1 , wherein the plastic fluidic portion comprises polypropylene.

19. The cartridge of claim 1 , wherein the plastic pneumatic portion comprises polypropylene.

20. The cartridge of claim 1 , wherein the plastic housing comprises acrylonitrile butadiene styrene.