Method for nuclei storage
View Patent ↗A method of performing data management in a high-speed data environment. The method includes collecting time-series information including multiple data types captured concurrently, and storing the collected time-series information in a process historian with organization, the organization occurring when the multiple data types are captured.
1. A method for storing cell nuclei comprising:
selectively lysing the cytoplasmic membranes and a minority proportion of the nuclear membranes present in a cellular sample to leave a majority proportion of the cell nuclei intact,
collecting the cellular sample on a solid support,
washing the solid support, and
storing the intact nuclei on the solid support at ambient temperature.
2. The method according to claim 1 , wherein the intact nuclei contains nucleic acid.
3. The method according to claim 1 , further comprising submerging the solid support in an organic solvent after washing the solid support and prior to storing the intact nuclei on the solid support at ambient temperature.
4. The method according to claim 1 , wherein the solid support is air dried prior to storing the intact nuclei on the solid support at ambient temperature.
5. The method according to claim 2 , wherein a lysis reagent is added to the solid support and subsequently dried in situ to preserve the nucleic acid.
6. The method according to claim 5 , wherein the lysis reagent comprises an anionic surfactant or detergent.
7. The method according to claim 6 , wherein the anionic surfactant is sodium dodecyl sulfate (SDS).
8. The method according to claim 1 , wherein the cellular sample has a volume greater than 1 ml.
9. The method according to claim 1 , wherein the cellular sample has a volume greater than 5 ml.
10. The method according to claim 1 , wherein the cellular sample has a volume greater than 10 ml.
11. The method according to claim 1 , wherein the solid support is a permeable membrane.
12. The method according to claim 1 , wherein the solid support is selected from the group consisting of polyester membrane, polyamide membrane, polycarbonate membrane and cellulose membrane.
13. The method according to claim 1 , wherein the solid support is a polyester membrane.
14. The method according to claim 1 , wherein the solid support is attached to a pipette tip.
15. The method according to claim 14 , wherein the solid support is a polyester membrane.
16. The method according to claim 14 , wherein the pipette tip comprises a microfilament weave.
17. The method according to claim 2 , further comprising recovering the nucleic acid from the nuclei after storing the intact nuclei on the solid support at ambient temperature.
18. The method according to claim 17 , wherein the nucleic acid is recovered by lysing the nuclei on the membrane.
19. The method according to claim 18 , wherein the membrane undergoes passive washing with a wash buffer.