AXMI422 toxin gene and methods for its use
View Patent ↗Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2-3, or the nucleotide sequence set forth in SEQ ID NO: 1, as well as variants and fragments thereof.
1. A construct comprising a heterologous promoter operably linked to a nucleotide sequence encoding an amino acid sequence having pesticidal activity, wherein said nucleotide sequence is selected from the group consisting of:
a) the nucleotide sequence set forth in SEQ ID NO:1; and
b) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of any of SEQ ID NO:2-3.
2. The construct of claim 1 , wherein said nucleotide sequence has been optimized for expression in a plant.
3. The construct of claim 1 , wherein said promoter is capable of directing expression of said nucleotide sequence in a plant cell.
4. A vector comprising the construct of claim 1 .
5. The vector of claim 4 , further comprising a nucleic acid molecule encoding a heterologous polypeptide.
6. A host cell that contains the construct of claim 1 .
7. The host cell of claim 6 that is a bacterial host cell.
8. The host cell of claim 6 that is a plant cell.
9. A transgenic plant comprising the host cell of claim 8 .
10. The transgenic plant of claim 9 , wherein said plant is selected from the group consisting of maize, sorghum, wheat, cabbage, sunflower, tomato, crucifers, peppers, potato, cotton, rice, soybean, sugarbeet, sugarcane, tobacco, barley, and oilseed rape.
11. A transgenic seed comprising the construct of claim 1 .
12. A recombinant polypeptide with pesticidal activity, wherein said polypeptide comprises a heterologous leader sequence or a transit peptide operably linked to
a polypeptide comprising the amino acid sequence of any of SEQ ID NO:2-3.
13. The polypeptide of claim 12 further comprising heterologous amino acid sequences.
14. A composition comprising the polypeptide of claim 12 .
15. The composition of claim 14 , wherein said composition is selected from the group consisting of a powder, dust, pellet, granule, spray, emulsion, colloid, and solution.
16. The composition of claim 14 , wherein said composition is prepared by desiccation, lyophilization, homogenization, extraction, filtration, centrifugation, sedimentation, or concentration of a culture of bacterial cells.
17. The composition of claim 14 , comprising from about 1% to about 99% by weight of said polypeptide.
18. A plant or plant cell having stably incorporated into its genome a DNA construct comprising a nucleotide sequence that encodes a protein having pesticidal activity, wherein said nucleotide sequence is selected from the group consisting of:
a) the nucleotide sequence set forth in SEQ ID NO:1; and
b) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of any of SEQ ID NO:2-3.