High speed, high resolution compositions, methods and kits for capillary electrophoresis
View Patent ↗The invention provides compositions, methods and kits for high speed, high resolution of analytes by capillary electrophoresis starting with uncoated capillaries. The compositions comprise a sieving component, comprising a non-crosslinked acrylamide polymer, and a surface interaction component, comprising at least one uncharged and non-crosslinked water-soluble silica-adsorbing polymer. Methods for employing the novel compositions in capillary electrophoresis are provided. Kits comprising the novel compositions for use in the novel methods are also provided.
1. A composition for separating analytes by capillary electrophoresis comprising:
a sieving component comprising a non-crosslinked acrylamide polymer having a weight average molecular weight between about 3,000,000 Da and less than or equal to about 6,400,000 Da; and
a surface interaction component comprising one or more non-crosslinked polymers selected from the group consisting of poly(meth) acrylamide, N,N-disubstituted polyacrylamide and N-substituted polyacrylamide, wherein the N-substituents are selected from the group consisting of C1 to C3 alkyl, halo-substituted C1 to C3 alkyl, methoxy-substituted C1 to C3 alkyl, and hydroxyl-substituted C1 to C3 alkyl;
wherein the surface interaction component is present in the composition at a concentration in the range of about 0.01% to about 1% w/w;
wherein the sieving component and the surface interaction component differ in polymer chemical composition; and
wherein the composition does not include a crosslinked polymer gel.
2. The composition of claim 1 , further comprising at least one denaturant.
3. The composition of claim 2 , wherein the at least one denaturant is selected from the group consisting of at least one of formamide, urea, and 2-pyrollidinone.
4. The composition of claim 3 , wherein the at least one denaturant comprises urea.
5. The composition of claim 1 wherein the non-crosslinked acrylamide polymer of the sieving component is polyacrylamide.
6. The composition of claim 5 , wherein the non-crosslinked polyacrylamide polymer is linear polyacrylamide.
7. A composition for separating analytes by capillary electrophoresis comprising:
a sieving component comprising a non-crosslinked acrylamide polymer having a weight average molecular weight between about 3,000,000 Da and less than or equal to about 6,400,000 Da;
a surface interaction component comprising poly(N,N-dimethylacrylamide);
wherein the sieving component and the surface interaction component differ in polymer chemical composition;
wherein the surface interaction component is present in the composition at a concentration in the range of about 0.01% to about 1% w/w; and
wherein the composition does not include a crosslinked polymer gel.
8. A capillary electrophoresis element comprising:
an uncoated capillary;
a composition for separating analytes located within the uncoated capillary, the composition comprising: a sieving component comprising an acrylamide polymer having a weight average molecular weight between about 3,000,000 Da and less than or equal to about 6,400,000 Da; and a surface interaction component;
wherein the surface interaction component comprises a solution of one or more polymers selected from the group consisting of poly(meth) acrylamide, N,N-disubstituted polyacrylamide and N-substituted polyacrylamide, wherein the N-substituents are selected from the group consisting of C1 to C3 alkyl, halo-substituted C1 to C3 alkyl, methoxy-substituted C1 to C3 alkyl, and hydroxyl-substituted C1 to C3 alkyl;
wherein the sieving component and the surface interaction component differ in polymer chemical composition;
wherein the surface interaction component is present in the composition at a concentration in the range of about 0.01% to about 1% w/w; and
wherein the composition within the capillary electrophoresis element does not include a crosslinked polymeric gel.
9. The capillary electrophoresis element of claim 8 , wherein the surface interaction component polymer is poly(N,N-dimethylacrylamide).
10. The capillary element of claim 8 , wherein the capillary comprises silica, fused silica, quartz, silicate-based glass, phosphate glass, alumina-containing glass.
11. The capillary element of claim 8 , wherein the capillary is a plastic channel capillary.
12. A kit for separating analytes by capillary electrophoresis comprising a composition comprising a sieving component comprising an non-crosslinked acrylamide polymer having a weight average molecular weight between about 3,000,000 Da and less than or equal to about 6,400,000 Da;
a surface interaction component comprising one or more non-crosslinked polymers selected from the group consisting of N,N-disubstituted polyacrylamide and N-substituted polyacrylamide,
wherein the N-substituents are selected from the group consisting of C1 to C3 alkyl, halo-substituted C1 to C3 alkyl, methoxy-substituted C1 to C3 alkyl, and hydroxyl-substituted C1 to C3 alkyl;
wherein the sieving component and the surface interaction component differ in polymer chemical composition;
wherein the composition does not include a crosslinked polymer gel.
13. The kit of according to claim 12 , wherein the surface interaction component is poly(N,N-dimethylacrylamide).
14. The kit of claim 12 , wherein the composition further comprises a denaturant.
15. The kit of claim 14 , wherein the denaturant is selected from at least one of formamide, urea, and 2-pyrollidinone.
16. The kit of claim 12 wherein the non-crosslinked acrylamide polymer of the sieving component is polyacrylamide.
17. The kit of claim 16 , wherein the non-crosslinked polyacrylamide polymer of the sieving component is linear polyacrylamide.