Dig-303 insecticidal Cry toxins
DIG-303 insecticidal toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.
1. An isolated, treated, or formulated DIG-303 insecticidal toxin polypeptide comprising an amino acid sequence selected from the group consisting of
(a) residues 11 to 685 of SEQ ID NO:2, comprising a C-terminal protoxin portion of a Cry toxin other than DIG-303; and
(b) residues 11 to 685 of SEQ ID NO:2 with up to 20 amino acid substitutions, deletions, or modifications that do not adversely affect expression or activity of the toxin encoded by SEQ ID NO:2.
2. The isolated, treated, or formulated polypeptide of claim 1 comprising an amino acid sequence of SEQ ID NO:2.
3. The isolated, treated, or formulated polypeptide of claim 1 , wherein the C-terminal protoxin portion comprises the C-terminal protoxin portion of Cry1Ab.
4. A method for controlling a pest population comprising contacting said population with a pesticidally effective amount of the polypeptide of claim 1 .
5. The polypeptide of claim 1 having activity against a coleopteran pest or Diamondback moth.
6. The polypeptide of claim 1 having activity against Colorado potato beetle or Diamondback moth.
7. A composition comprising the polypeptide of claim 1 .
8. The composition of claim 7 , wherein the composition is a sprayable protein composition, encapsulated protein composition, or bait matrix that comprises the formulated DIG-303 insecticidal toxin.
9. A nucleic acid construct comprising a heterologous nucleic acid sequence that is recombinantly linked to a sequence encoding the polypeptide of claim 1 .
10. The nucleic acid construct of claim 9 , wherein the heterologous nucleic acid sequence is a promoter sequence capable of driving expression in a plant.
11. The nucleic acid construct of claim 9 , wherein the sequence encoding the polypeptide is codon-optimized for expression in a plant.
12. The nucleic acid construct of claim 10 , wherein the promoter is capable of driving expression in corn and the sequence encoding the polypeptide is codon optimized for expression in corn.
13. The nucleic acid construct of claim 9 , wherein the sequence encoding the polypeptide comprises SEQ ID NO:1 or SEQ ID NO:3.
14. The nucleic acid construct of claim 12 , wherein the construct is a vector and the vector comprises SEQ ID NO:3.
15. The nucleic acid construct of claim 10 , wherein the promoter is capable of driving expression in potato and the sequence encoding the polypeptide is codon optimized for expression in potato.
16. A transgenic plant comprising the nucleic acid construct of claim 10 stably incorporated into its genome.
17. A method for protecting a plant from a pest comprising introducing into said plant the construct of claim 9 .