Microscope apparatus
View Patent ↗A microscope apparatus according to the present invention includes a bright-field illumination optical system and a fluorescence illumination optical system that respectively radiate illumination light and excitation light onto a sample, an observation optical system that observes observation light from the sample, and a controller that controls the fluorescence illumination optical system so that the excitation light is radiated onto the sample when the irradiation of the illumination light on the sample is prevented by an irradiation preventing portion of the bright-field illumination optical system and so that the irradiation of the excitation light on the sample is prevented when the irradiation of the illumination light on the sample is not prevented by the irradiation preventing portion.
1. A microscope apparatus comprising:
a bright-field illumination optical system that radiates illumination light onto a sample for performing bright-field observation of the sample;
a fluorescence illumination optical system that radiates excitation light onto the sample for performing fluorescence observation of the sample, the fluorescence illumination optical system including a plurality of excitation light sources having different wavelengths for performing a plurality of kinds of the fluorescence observation;
an observation optical system that observes observation light from the sample; and
a controller that controls at least the fluorescence illumination optical system,
wherein the bright-field illumination optical system includes:
a shutter that prevents irradiation of the sample with the illumination light; and
a shutter detecting portion that detects insertion of the shutter into an optical path of the bright-field illumination optical system and withdrawal of the shutter from the optical path of the bright-field illumination optical system, and that detects switching of the plurality of excitation light sources,
wherein when the controller detects, through the shutter detecting portion, that the shutter is inserted into optical path of the bright-field illumination optical system, the controller causes one of the plurality of excitation light sources that corresponds to information detected by the shutter detecting portion to radiate the excitation light onto the sample, and
wherein when the controller detects, through the shutter detecting portion, that the shutter is withdrawn from the optical path of the bright-field illumination optical system, the controller prevents the irradiation of the excitation light on the sample by the fluorescence illumination optical system.
2. The microscope apparatus according to claim 1 , wherein the shutter detecting portion includes a sensor that detects a marker provided on the shutter.
3. The microscope apparatus according to claim 2 , wherein the shutter includes a plurality of markers corresponding to the plurality of excitation light sources respectively, and
wherein the controller causes one of the plurality of excitation light sources that corresponds to information about the marker detected by the sensor to radiate the excitation light onto the sample.
4. The microscope apparatus according to claim 3 , wherein the shutter further includes a marker corresponding to the bright-field observation.
5. The microscope apparatus according to claim 2 , wherein the sensor is a magnetic sensor.
6. The microscope apparatus according to claim 1 , wherein the shutter detecting portion includes an optical sensor that detects the illumination light from the bright-field illumination optical system.
7. The microscope apparatus according to claim 1 , further comprising at least one fluorescence cube that performs reflection of excitation light and transmission of fluorescence light,
wherein when the controller detects, through the shutter detecting portion, that the shutter is inserted into the optical path of the bright-field illumination optical system, the controller causes the fluorescence cube to be inserted into an optical path of the fluorescence illumination optical system, and
wherein when the controller detects, through the shutter detecting portion, that the shutter is withdrawn from the optical path of the bright-field illumination optical system, the controller causes the fluorescence cube to be withdrawn from the optical path of the fluorescence illumination optical system.
8. The microscope apparatus according to claim 7 , wherein the at least one fluorescence cube is a plurality of fluorescence cubes which correspond to the plurality of excitation light sources respectively, and
wherein when the controller detects, through the shutter detecting portion, that the shutter is inserted into the optical path of the bright-field illumination optical system, the controller causes one of the plurality of fluorescence cubes that corresponds to information detected by the shutter detecting portion to radiate the excitation light onto the sample.