CONFOCAL LASER SCANNING MICROSCOPE
A confocal laser scanning microscope includes a laser light source configured to emit laser light as illumination light, an objective, a scanner placed in an optical path between the laser light source and the objective for scanning the sample with the illumination light, and a stop placed on a pupil plane of the objective or in its vicinity, or on a plane that is optically conjugate with the pupil plane of the objective or in its vicinity, configured to block, of light from the sample, light that is a regular reflection of the illumination light cast on the sample. The stop is placed on a plane that is conjugate with the pupil plane of the objective or in its vicinity in an optical path between the laser light source and the scanner.
1 . A confocal laser scanning microscope comprising:
a laser light source configured to emit laser light as illumination light;
an objective configured to cast the illumination light on a sample and to take in light from the sample;
a scanner placed in an optical path between the laser light source and the objective for scanning the sample with the illumination light; and
a stop placed on a pupil plane of the objective or in a vicinity of the pupil plane, or on a plane that is optically conjugate with the pupil plane of the objective or in a vicinity of the plane that is optically conjugate with the pupil plane, configured to block, of the light from the sample, light that is a regular reflection of the illumination light cast on the sample,
wherein
the stop is placed on a plane that is conjugate with the pupil plane of the objective or in a vicinity of the plane that is optically conjugate with the pupil plane in an optical path between the laser light source and the scanner.
2 . The confocal laser scanning microscope according to claim 1 , wherein
the stop is a stop in which an aperture is formed so that an area that is symmetric with the aperture with respect to an axial principal ray of the illumination light becomes a light blocking portion that blocks light.
3 . The confocal laser scanning microscope according to claim 2 , further comprising
a first stop moving mechanism configured to move the stop so as to change an orientation of the aperture with respect to the axial principal ray of the illumination light.
4 . The confocal laser scanning microscope according to claim 1 , wherein:
the stop is a stop in which a plurality of apertures that are switched and placed in the optical path between the laser light source and the scanner by a movement of the stop are formed, and
each of the plurality of apertures is formed so that, when each of the plurality of apertures is placed in the optical path, an area that is symmetric with the aperture with respect to an axial principal ray of the illumination light becomes a light blocking portion that blocks light.
5 . The confocal laser scanning microscope according to claim 4 , wherein
orientations of the plurality of the apertures with respect to the axial principal ray of the illumination light are different from each other, when placed in the optical path.
6 . The confocal laser scanning microscope according to claim 4 , further comprising
a first stop moving mechanism configured to move the stop so that an aperture selected from the plurality of apertures is placed in the optical path between the laser light source and the scanner.
7 . The confocal laser scanning microscope according to claim 1 , further comprising
a second stop moving unit configured to move the stop in a direction orthogonal to an axial principal ray of the illumination light.
8 . The confocal laser scanning microscope according to claim 1 , wherein:
the stop includes a plurality of light blocking plates provided so as to move in different directions that are orthogonal to an axial principal ray of the illumination light, and
the confocal laser scanning microscope further comprises a light blocking plate moving mechanism configured to move the plurality of light blocking plates so that an area that is symmetric with the aperture of the stop with respect to the axial principal ray of the illumination light becomes a blocking portion that blocks light.
9 . The confocal laser scanning microscope according to claim 1 , wherein
the stop is placed detachably with respect to the optical path between the laser light source and the scanner.