Blue transgenic fluorescent ornamental fish
View Patent ↗The present invention relates to transgenic blue ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.
1. A transgenic tetra comprising a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the tetra comprises the “Blue tetra 1 transformation event,” sperm comprising the Blue tetra 1 transformation event having been deposited as ECACC accession no. 14103001.
2. The transgenic tetra of claim 1 , further defined as a fertile, transgenic tetra.
3. The transgenic tetra of claim 1 , further defined as a transgenic White Tetra.
4. The transgenic tetra of claim 1 , wherein the fish is homozygous for the integrated expression cassette.
5. The transgenic tetra of claim 1 , wherein the fish is heterozygous for the integrated expression cassette.
6. A method of producing a transgenic tetra comprising:
(a) obtaining a tetra that comprises a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the tetra comprises the “Blue tetra 1 transformation event,” sperm comprising the Blue tetra 1 transformation event having been deposited as ECACC accession no. 14103001; and
(b) breeding the obtained tetra with a second tetra to provide a transgenic tetra comprising the Blue tetra 1 transformation event.
7. The method of claim 6 , wherein the second tetra is a non-transgenic tetra.
8. A progeny of a transgenic tetra of claim 1 that comprises a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the tetra and progeny exhibit fluorescence and comprise the “Blue tetra 1 transformation event,” sperm comprising the Blue tetra 1 transformation event having been deposited as ECACC accession no. 14103001.
9. The progeny fish of claim 8 , further defined as a fertile, transgenic tetra.
10. The progeny fish of claim 8 , further defined as a transgenic White Tetra.
11. The progeny fish of claim 8 , wherein the fish is homozygous for the integrated expression cassette.
12. The progeny fish of claim 8 , wherein the fish is heterozygous for the integrated expression cassette.
13. A method of producing a transgenic fish comprising:
(a) obtaining a transgenic fish in accordance with claim 8 ; and
(b) breeding the obtained fish with a second fish to provide a transgenic fish comprising the Blue tetra 1 transformation event.
14. The method of claim 13 , wherein the second fish is a non-transgenic fish.