Enzymatically polymerized gelling dextrans
View Patent ↗Compositions are disclosed herein comprising dextran that comprises (i) 87-93 wt % glucose linked at positions 1 and 6; (ii) 0.1-1.2 wt % glucose linked at positions 1 and 3; (iii) 0.1-0.7 wt % glucose linked at positions 1 and 4; (iv) 7.7-8.6 wt % glucose linked at positions 1, 3 and 6; and (v) about 0.4-1.7 wt % glucose linked at (a) positions 1, 2 and 6, or (b) positions 1, 4 and 6. Aqueous forms of this composition have enhanced viscosity profiles. Further disclosed are methods of using compositions comprising dextran, such as increasing the viscosity of an aqueous composition. Enzymatic reactions for producing dextran are also disclosed.
1. A composition comprising an isolated dextran, wherein said dextran comprises:
(i) about 87-93 wt % glucose linked at positions 1 and 6;
(ii) about 0.1-1.2 wt % glucose linked at positions 1 and 3;
(iii) about 0.1-0.7 wt % glucose linked at positions 1 and 4;
(iv) about 7.7-8.6 wt % glucose linked at positions 1, 3 and 6; and
(v) about 0.4-1.7 wt % glucose linked at:
(a) positions 1, 2 and 6, or
(b) positions 1, 4 and 6;
wherein the weight-average molecular weight (Mw) of said dextran is about 50-200 million Daltons, the z-average radius of gyration of said dextran is about 200-280 nm, and the dextran is a product of an isolated glucosyltransferase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:1 or SEQ ID NO:2.
2. The composition of claim 1 , wherein the dextran comprises:
(i) about 89.5-90.5 wt % glucose linked at positions 1 and 6;
(ii) about 0.4-0.9 wt % glucose linked at positions 1 and 3;
(iii) about 0.3-0.5 wt % glucose linked at positions 1 and 4;
(iv) about 8.0-8.3 wt % glucose linked at positions 1, 3 and 6; and
(v) about 0.7-1.4 wt % glucose linked at:
(a) positions 1, 2 and 6, or
(b) positions 1, 4 and 6.
3. The composition of claim 1 , wherein the dextran comprises chains iteratively linked together within a branching structure, wherein said chains are similar in length and comprise substantially alpha-1,6-glucosidic linkages.
4. The composition of claim 3 , wherein the average length of the chains is about 10-50 monomeric units.
5. The composition of claim 1 , wherein the dextran is a product of an isolated glucosyltransferase enzyme comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:1 or SEQ ID NO:2.
6. The composition of claim 1 , wherein the composition is an aqueous composition having a viscosity of at least about 25 centipoise.
7. The composition of claim 1 , wherein the Mw of the dextran is about 80-120 million Daltons.
8. The composition of claim 1 , wherein the z-average radius of gyration of said dextran is about 230-250 nm.
9. The composition of claim 1 , wherein the composition is in the form of a food product, personal care product, pharmaceutical product, household product, or industrial product.
10. The composition of claim 9 , wherein the composition is in the form of a confectionery.
11. A method for increasing the viscosity of an aqueous composition, the method comprising:
contacting dextran according to claim 1 with the aqueous composition, wherein the viscosity of the aqueous composition is increased by said dextran compared to the viscosity of the aqueous composition before the contacting step.
12. A method of treating a material, said method comprising:
contacting the material with an aqueous composition comprising dextran according to claim 1 .