Thermal cycling
View Patent ↗A processing apparatus includes a carrier receiving region configured to receive a sample carrier with at least one channel that carries at least one sample. The apparatus further includes a thermal control device configured to thermal cycle the sample carrier when the sample carrier is installed in the carrier receiving region, thereby thermal cycling the sample carried therein. The apparatus further includes a thermal control system configured to control the temperature control device based on a predetermined set of target sample temperatures and a temperature map, which maps the predetermined set of target sample temperatures to a set of temperatures of the temperature control device. The set of temperatures of the temperature control device is different from the predetermined set of target sample temperatures, and the set of temperatures of the temperature control device thermal cycle the sample carrier with the temperatures of the predetermined set of target sample temperatures.
1. A system, comprising:
a thermal control system configured to control a temperature of a temperature control device based on a set of target temperatures and a corresponding timing sequence;
a calibration carrier, including:
a polymerase chain reaction region configured to be in thermal contact with the temperature control device;
a plurality of channels configured to process samples both individually and in parallel, wherein a portion of each channel is in the polymerase chain reaction region; and
a plurality of thermocouples, each channel of the plurality of channels in thermal communication with a different one of the thermocouples of the plurality of thermocouples at the portion of the respective channel of the plurality of channels that is located in the polymerase chain reaction region,
wherein each thermocouple generates a signal that maps a temperature of the portion of its respective channel to the set of target temperatures and the corresponding timing sequence, and
wherein the temperature of the portion is one of a denaturing temperature, an annealing temperature or an extending temperature of a replication of a polymerase chain reaction; and
a carrier receiving region configured to separately receive only one of the calibration carrier or a sample carrier at a given time.
2. The system of claim 1 , wherein the calibration carrier is structurally configured for installation into a carrier receiving region of a sample processing apparatus.
3. The system of claim 1 , wherein the calibration carrier includes a peripheral region of the polymerase chain reaction region configured with an interface for an external temperature sensor.
4. The system of claim 3 , wherein the signal maps the temperature of the portion to a temperature of the peripheral region.
5. The system of claim 3 , wherein the signal maps a predetermined temperature of the portion to a first applied temperature at a first time.
6. The system of claim 5 , wherein the signal maps the predetermined temperature of the portion to a different applied temperature at a different time.
7. The system of claim 6 , wherein the predetermined temperature is a denaturing temperature of 94±0.25° C.
8. The system of claim 6 , wherein the predetermined temperature is an annealing temperature of 56±0.25° C.
9. The system of claim 6 , wherein the predetermined temperature is an extending temperature of 70±0.25° C.
10. The system of claim 1 , wherein the calibration carrier and the sample carrier have a same thermal property and a same physical geometry.
11. The system of claim 1 , further comprising:
one or more processing stations configured to carry out one or more of extraction or purification of DNA fragments from a sample, labeling of extracted fragments with different fluorescent dyes, replication of the labeled fragments, separation of the replicated labeled fragments, and analysis of the separated fragments.
12. The system of claim 1 ,
wherein the temperature control device controls the temperature applied to the calibration carrier.