Determining tumor load and biallelic mutation in patients with CALR mutation using peripheral blood plasma
Compositions and fragment length analysis methods are provided for detecting CALR mutations and determining tumor load in patients with myeloproliferative neoplasms.
1. A method for treating a patient for a myeloproliferative neoplasm (MPN), the method comprising:
(a) testing the patient by:
obtaining a sample of peripheral blood plasma from the patient;
extracting cell-free DNA from the sample;
performing fragment length analysis of calreticulin human gene (CALR) in the extracted cell-free DNA; and
determining tumor load and biallelic mutation in calreticulin human gene (CALR); and
(b) administering an anti-MPN therapeutic agent to the patient.
2. The method of claim 1 , wherein the fragment length analysis comprises a step of labeling CALR fragments with one or more fluorescent dyes, amplifying the labeled fragments using polymerase chain reaction (PCR), separating the labeled fragments by size using capillary electrophoresis, and analyzing the data using software to determine the size of the amplified labeled fragments and genotype.
3. The method of claim 1 , wherein the fragment length analysis comprises a step of amplifying a fragment of CALR gene by PCR.
4. The method of claim 3 , wherein the amplified fragment comprises at least a portion of CALR exon 9.
5. The method of claim 3 , wherein the amplification is performed with primers specific to CALR exon 9 and wherein at least one of the primers is labeled with a fluorescent dye.
6. The method of claim 3 , wherein the amplification is performed with the following primers: GGC AAG GCC CTG AGG TGT (SEQ ID NO. 1) and GGC CTC AGT CCA GCC CTG (SEQ ID NO. 2).
7. The method of claim 3 , wherein the amplification is performed with primers specific to CALR and wherein at least one of the primers is labeled with a fluorescent dye.
8. The method of claim 1 , wherein testing further comprises bidirectional sequencing of at least a portion of CALR.
9. The method of claim 1 , wherein MTN is selected from the group consisting of chronic myelogenous leukemia, polycythemia vera, primary myelofibrosis, essential thrombocythemia, chronic neutrophilic leukemia and chronic eosinophilic leukemia.
10. The method of claim 1 , wherein testing further comprises determining mutations in Janus kinase 2 (JAK-2) and myeloproliferative leukemia protein (MPL).
11. The method of claim 1 , wherein the biallelic mutation in CALR is indicated if a mutant peak is at least 55% of a normal control peak.
12. A method of treating a patient for a myeloproliferative neoplasm (MPN), the method comprising:
(a) testing the patient by:
obtaining a sample of peripheral blood plasma from the patient;
extracting cell-free DNA from the sample;
amplifying a fragment of CALR within the cell-free DNA by a PCR;
separating the amplified CALR fragments by size;
determining the size of the amplified CALR fragments;
generating an electropherogram and comparing peak intensities of mutant and wild-type peak to determine a mutation rate, wherein a mutation rate that exceeds a threshold indicates a biallelic mutation in CALR; and
(b) administering a treatment for MPN.
13. The method of claim 12 , wherein PCR is conducted with primers specific for CALR exon 9.
14. The method of claim 12 , wherein the PCR is conducted with a composition comprising the primer with SEQ ID NO. 1 and primer with SEQ ID NO. 2.
15. The method of claim 12 , wherein the amplified CALR fragments are separated by capillary electrophoresis and wherein the amplified fragments are labeled with a fluorescent dye.
16. The method of claim 12 , wherein MPN is selected from the group consisting of chronic myelogenous leukemia, polycythemia vera, primary myelofibrosis, essential thrombocythemia, chronic neutrophilic leukemia and chronic eosinophilic leukemia.
17. The method of claim 12 , wherein the treatment is selected from the group consisting of blood transfusions, chemotherapeutic agents, radiation therapy and stem cell transplants.
18. The method of claim 1 , wherein determining tumor load and biallelic mutation comprises:
generating an electropherogram; and
comparing peak intensities of mutant and wild-type peaks within the electropherogram to determine a mutant rate.
19. The method of claim 12 , wherein the threshold is exceeded when the mutant peak is at least 55% of the wild-type peak.