Glycosaminoglycan compositions in combination with stem cells
A pharmaceutical preparation for treating connective tissue damage in man and in animals, comprising a therapeutically effective amount of a glycosaminoglycan composition comprising chondroitin sulfate and hyaluronan, in combination with isolated stem cells. Methods of use and kits containing the glycosaminoglycan composition and materials for isolating stem cells and for treating connective tissue damage and repair of cartilage in man and in animals are also provided.
1. A method of cryopreserving isolated stem cells comprising combining isolated stem cells with a composition comprising chondroitin sulfate and hyaluronan in a container, and cryopreserving the combination in the container.
2. The method of claim 1 , wherein the composition further comprises N-acetyl D-glucosamine.
3. The method of claim 1 , wherein said chondroitin sulfate is a mixture of chondroitin 4-sulfate and chondroitin 6-sulfate.
4. The method of claim 1 , wherein the chondroitin sulfate is in a concentration of about 50-100 mg/ml.
5. The method of claim 1 , wherein the chondroitin sulfate is in a concentration of about 100 mg/ml.
6. The method of claim 1 , wherein the hyaluronan is in a concentration of about 1-5 mg/ml.
7. The method of claim 1 , wherein the hyaluronan is in a concentration of about 5 mg/ml.