Methods and compositions for identifying Verticillium wilt resistant sunflower plants
The present invention relates to methods for identifying sunflower lines having increased resistance to Verticillium wilt, and identification of genetic markers linked to gene(s) conditioning such increased disease resistance. The present invention also relates to methods of breeding sunflower plants from lines having increased Verticillium wilt resistance by marker-assisted selection, compositions including nucleic acid probes or primers which are useful for such marker assisted selection, and plants and plant parts produced by such methods.
1. A method of identifying a sunflower plant that displays resistance to Verticillium dahlia wilt, the method comprising the steps of: a) genotyping a population of sunflower plants, from a segregating population derived from sunflower variety CN8861R, with at least one sunflower genomic nucleic acid marker associated with an allele for Verticillium dahlia wilt resistance, wherein said allele comprises at least a first genetic marker genetically linked to a QTL that confers resistance to Verticillium dahlia wilt; b) selecting at least a first sunflower plant comprising the genetic marker and the QTL that confers resistance to Verticillium dahlia wilt; wherein the QTL maps to a position between the sequence represented by SNP marker DHAI005760 and SNP marker DHAI006271 which map to approximately 46.5 cM and 54.7 cM on the genetic map of the linkage group termed sunflower chromosome 10, and c) crossing the sunflower plant selected in step (b) with another sunflower plant; wherein selecting the first sunflower plant further comprises selecting the plant based on the presence of a plurality of genetic markers that map to a position between the sequence represented by SNP marker DHAI005760 and SNP marker DHAI006271 which map to approximately 46.5 cM and 54.7 cM on the genetic map of the linkage group termed sunflower chromosome 10, and wherein the genetic markers are selected from the group consisting of markers DHAI006271, DHAI000041, and DHAI005760, comprising a single nucleotide polymorphism of one of SEQ ID NOs:1-3.
2. A method of identifying a sunflower plant that displays resistance to Verticillium dahlia wilt, the method comprising the steps of: a) genotyping a population of sunflower plants, from a segregating population derived from sunflower variety CN8861R, with at least one sunflower genomic nucleic acid marker associated with an allele for Verticillium dahlia wilt resistance, wherein said allele comprises least a first genetic marker genetically linked to a QTL that confers resistance to Verticillium dahlia wilt; b) selecting at least a first sunflower plant comprising the genetic marker and the QTL that confers resistance to Verticillium dahlia wilt; wherein the QTL maps to a position between the sequence represented by SNP marker DHAI005760 and SNP marker DHAI006271 which map to approximately 46.5 cM and 54.7 cM on the genetic map of the linkage group termed sunflower chromosome 10; and c) crossing the sunflower plant selected in step (b) with another sunflower plant; wherein the genetic marker is DHAI005760, DHAI000041, or DHAI006271.