Low sugar sperm media and compositions
The present invention relates to compositions comprising low sugar media, the methods of using these compositions to reduce trauma and stress on processed animal sperm, the resulting sperm and embryo products, and the methods of use of these products to increase the quality, quantity and viability of progeny and improved rates of births in animals.
1. A method of processing sperm comprising the steps of:
forming a stream comprising sperm and a sheath fluid;
determining a property of said sperm in said stream;
selecting sperm having a property of interest; and
collecting said sperm having said property of interest in a catch media that does not contain a sugar additive, wherein said catch media comprises egg yolk at a concentration of about 16.7% and wherein said catch media is frozen and thawed prior to said step of collecting.
2. The method of claim 1 , wherein the step of selecting sperm having a property of interest comprises photo-damaging said sperm having said property of interest.
3. The method of claim 1 , wherein the step of selecting sperm having a property of interest comprises isolating said sperm having said property of interest.
4. The method of claim 1 , wherein said sheath fluid or said catch media comprise one or more organic stress reducing agents (OSRs).
5. The method of claim 4 , wherein the one or more OSRs is selected from the following: catalase, SOD, an SOD mimic, glutathione, glutathione reductase, glutathione peroxidase, pyruvate, mercaptoethanol, BHT, lipoic acid, flavins, quinines, vitamin K (and related vitamers), vitamin B12, vitamin B12 vitamers, vitamin E (and related vitamers), tocopherols, tocotrienols, α-tocopheryl, alpha ketoglutarate (AKG), malondialdehyde (MDA), asymmetric dimethylarginine (ADMA) and biologically active derivatives thereof.
6. The method of claim 5 , wherein the one or more OSRs is in the concentration range of 0.01 mg/ml to 5 mg/ml.
7. The method of claim 1 , further comprising the step of resuspending at least one of the one or more subpopulations in a resuspension media comprising 1 mM to 10 mM of a sugar additive.
8. The method of claim 7 , wherein said resuspension media comprises one or more OSRs.
9. The method of claim 1 , further comprising the step of fertilizing an egg with sperm having said property of interest or inseminating a female with sperm having said property of interest.