SSTR-TARGETED CONJUGATES ENCAPSULATED IN PARTICLES AND FORMULATIONS THEREOF
Particles, including nanoparticles and microparticles, and pharmaceutical formulations thereof, containing conjugates of an active agent such as a therapeutic, prophylactic, or diagnostic agent attached to a targeting moiety via a linker have been designed which can provide improved temporospatial delivery of the active agent and/or improved biodistribution. Methods of making the conjugates, the particles, and the formulations thereof are provided. Methods of administering the formulations to a subject in need thereof are provided, for example, to treat or prevent cancer or infectious diseases.
1 . A polymeric controlled release nanoparticle comprising a conjugate of a chemotherapeutic agent bound via a linker to a targeting moiety, said targeting moiety binds to cells of solid tumors to which the chemotherapeutic agent is to be delivered, wherein the polymeric nanoparticle is synthesized as a solid polymeric nanoparticle having a diameter of between about 10 nm to about 500 nm and wherein the conjugate is distributed in the solid polymeric nanoparticle and wherein, upon administration, the solid nanoparticle preferentially accumulates at sites of said solid tumors.
2 . The polymeric controlled release nanoparticle of claim 1 , wherein the linker is a cleavable linker.
3 . The polymeric controlled release nanoparticle of claim 1 , wherein each linker is independently selected from the group consisting of C 2 -C 30 carboxylic acids, C 2 -C 30 di-carboxylic acids and derivatives thereof.
4 . The polymeric controlled release nanoparticle of claim 3 , wherein the linker is a C 2 -C 30 carboxylic acid or a C 2 -C 30 di-carboxylic acid comprising an atom or group of atoms selected from the group consisting of —O—, —C(═O)—, —NR, —O—C(═O)—NR—, —S—, and —S—S—, wherein R is a linear or branched alkyl or heteroalkyl group.
5 . The polymeric controlled release nanoparticle of claim 1 , wherein the linker is selected from the group consisting of C 2 -C 30 carboxylic acids and di-carboxylic acids containing a dithio (—S—S—) group in the backbone.
6 . The polymeric controlled release nanoparticle of claim 1 , wherein the active agent is a small molecule, a protein, peptide, lipid, carbohydrate, sugar, nucleic acid, or combination thereof.
7 . The polymeric controlled release nanoparticle of claim 1 , wherein the active agent is targeted to a tyrosine kinase receptor.
8 . The polymeric controlled release nanoparticle of claim 1 , wherein the targeting moiety is selected from the group consisting of peptides and polypeptides, antibody mimetics, nucleic acids, glycoproteins, small molecules, carbohydrate, and lipids.
9 . The polymeric controlled release nanoparticle of claim 8 , wherein the targeting moiety targets a marker selected from the group consisting of CD19, CD70, CD56, PSMA, alpha integrin, CD22, CD138, EGFR, EphA2, AGS-5, Nectin-4, HER2, GPMNB, CD74, and Le.
10 . The polymeric controlled release nanoparticle of claim 8 , wherein the targeting moiety is selected from the group consisting of RGD, Cy5.5, somatostatin, octreotide, lancreotide, PSMA, or derivatives thereof.
11 . The polymeric controlled release nanoparticle of claim 1 , wherein the polymer is selected from the group consisting of hydrophobic polymers, hydrophilic polymers, and copolymers thereof.
12 . The polymeric controlled release nanoparticle of claim 11 , wherein the hydrophobic polymers are selected from the group consisting of polyhydroxyacids, polyhydroxyalkanoates, olycaprolactones, poly(orthoesters), polyanhydrides, poly(phosphazenes), poly(lactide-co-caprolactones), polycarbonates, polyesteramides, polyesters, and copolymers thereof.
13 . The polymeric controlled release nanoparticle of claim 11 , wherein the hydrophilic polymers are selected from the group consisting of polyalkylene glycols, polyalkylene oxides, poly(oxyethylated polyol), poly(olefinic alcohol), polyvinylpyrrolidone), poly(hydroxyalkylmethacrylamide), poly(hydroxyalkylmethacrylate), poly(saccharides), poly(hydroxy acids), poly(vinyl alcohol), and copolymers thereof.
14 . The polymeric controlled release nanoparticle of claim 11 , wherein the polymer is selected from the group consisting of poly(lactic acid), poly(glycolic acid), poly(lactic-co-glycolic acid), poly(ethylene oxide), poly(ethylene glycol), poly(propylene glycol), and copolymers thereof.
15 . The polymeric controlled release nanoparticle of claim 1 , wherein the particle has a diameter between 50 and 120 nm.
16 . The polymeric controlled release nanoparticle of claim 1 , wherein the polymer comprises two or more different polymers.
17 . The polymeric controlled release nanoparticle of claim 1 , wherein the conjugate is present in an amount between 0.1% and 10% (w/w) based upon the weight of the particle.
18 . The polymeric controlled release nanoparticle of claim 2 , wherein the a cleavable linker is selected from the group consisting of pH-sensitive linkers, protease cleavable peptide linkers, nuclease sensitive nucleic acid linkers, lipase sensitive lipid linkers, glycosidase sensitive carbohydrate linkers, hypoxia sensitive linkers, photocleavable linkers, heat-labile linkers, enzyme cleavable linkers (e.g., esterase cleavable linker), ultrasound-sensitive linkers, and x-ray cleavable linkers.
19 . A pharmaceutical composition comprising the polymeric controlled release nanoparticle of claim 1 and a pharmaceutically acceptable excipient.
20 . A method of reducing tumor volume in a subject in need thereof comprising administering a therapeutically effective amount of the composition of claim 19 .