Method for measuring hematocrit value of blood sample, method for measuring concentration of analyte in blood sample, sensor chip and sensor unit
View Patent ↗Provided is a sensor chip for electrochemically measuring a concentration of an analyte in a blood sample. In one embodiment of the sensor, the sensor chip includes a substrate, and a preliminary measurement analyzer and a hematocrit value analyzer disposed on the substrate. The preliminary measurement analyzer includes a preliminary working electrode and a preliminary counter electrode. The hematocrit value analyzer includes a working electrode and a counter electrode. An oxidant of a redox substance is disposed on the preliminary measurement analyzer and the counter electrode. A reductant of a redox substance is disposed on the working electrode.
1. A method of determining an amount of a component in blood using a biosensor,
the biosensor comprising:
a first electrode,
a second electrode,
an oxidant of a redox substance, and
a reductant of a redox substance,
wherein the reductant of a redox substance is disposed only on the first electrode, and
the oxidant of a redox substance is disposed only on the second electrode;
the method comprising:
applying a first voltage to the first electrode and the second electrode such that the first electrode acts as a working electrode and the second electrode acts as a counter electrode;
detecting a first current value generated by the application of the first voltage;
applying a second voltage to the second electrode such that the second electrode acts as a working electrode;
detecting a second current value generated by the application of the second voltage; and
calculating an amount of the component using the first current value and the second current value.
2. The method of claim 1 , wherein the first voltage is applied for a time of 0.01 to 10 seconds.
3. The method of claim 1 , wherein the second voltage is applied for a time of 0.1 to 10 seconds.
4. The method of claim 1 , wherein the amount of the component is calculated taking into account the amount of hematocrit present in the blood.
5. The method of claim 1 , wherein the first and second electrode comprise gold or palladium or carbon.
6. The method of claim 1 , wherein a redox enzyme is disposed on the second electrode.