Fungal Endophytes for Improved Crop Yields and Protection from Pests
The invention provides a synthetic combination of a crop and at least one fungal endophyte, wherein the crop is a host plant of the endophyte. Provided are also methods and compositions for producing such synthetic combinations. The endophyte reproduces and enhances the agronomic characteristics of the crop. Methods for inoculating the host plant with the endophyte, for propagating the host-endophyte combination, and for detecting the presence of the endophyte and of its metabolites within a host plant are also described.
1 .- 20 . (canceled)
21 . A method for improving drought resistance in a cotton plant, the method comprising:
contacting a cotton plant or a seed of said cotton plant with a formulation comprising purified filamentous, spore-forming, facultative fungal endophytes of at least one species, wherein the facultative fungal endophytes are present in the formulation in an amount effective to improve drought resistance of the cotton plant or a cotton plant grown from the seed compared to a reference cotton plant or a cotton plant grown from a reference seed.
22 . The method of claim 21 , wherein the formulation contains at least 100 (10̂2) spores/ml or 100,000 (10̂5) spores/g dry weight of the facultative fungal endophytes.
23 . The method of claim 21 , wherein the facultative fungal endophytes are present in the formulation in an amount effective to increase biomass.
24 .- 26 . (canceled)
27 . The method of claim 21 , wherein the facultative fungal endophytes are present in the formulation in an amount effective to reduce yield loss.
28 . The method of claim 27 , wherein yield loss is reduced by at least 5%.
29 . The method of claim 27 , wherein the reduction in yield loss results in a yield increase of at least 5%.
30 . The method of claim 21 , wherein enhanced resistance to drought stress is assessed by withholding water from 7-day old seedlings of the cotton plant grown in the greenhouse, wherein the seedlings have increased time to wilt as compared to a seedling grown from a reference seed.
31 . A synthetic combination of a cotton seed and purified filamentous, spore-forming, facultative fungal endophytes of at least one species, wherein the facultative fungal endophytes are present in the formulation in an amount effective to improve drought resistance of the cotton plant grown from the seed compared to a plant grown from a reference seed.
32 . The synthetic combination of claim 31 , wherein the facultative fungal endophytes are present at a concentration of at least 100 (10̂2) spores/seed on the surface of the seed.
33 . The synthetic combination of claim 31 , wherein the facultative fungal endophytes are present in the formulation in an amount effective to increase biomass.
34 .- 36 . (canceled)
37 . The synthetic combination of claim 31 , wherein the facultative fungal endophytes are present in the formulation in an amount effective to reduce yield loss.
38 . The synthetic combination of claim 37 , wherein yield loss is reduced by at least 5%.
39 . The synthetic combination of claim 37 , wherein the reduction in yield loss results in a yield increase of at least 5%.
40 . The synthetic combination of claim 31 , wherein enhanced resistance to drought stress is assessed by withholding water from 7-day old seedlings of the cotton plant grown in the greenhouse, wherein the seedlings have increased time to wilt as compared to a seedling grown from a reference seed.