Biomarker profiles for predicting outcomes of cancer therapy with ERBB3 inhibitors and/or chemotherapies
Provided are methods for optimizing therapy of, treating a patient having, or selecting (identifying) patients who will benefit from treatment for, a cancer (e.g., a non-hematological cancer; e.g., a gynecological cancer). The methods comprise determining whether the patient will benefit from treatment with an ErbB3 inhibitor (e.g., an anti-ErbB3 antibody), with or without either a taxane or an aromatase inhibitor, or with a taxane or an aromatase inhibitor in the absence of an ErbB3 inhibitor, based on levels of particular biomarkers and combinations of biomarkers measured in a biological sample obtained from the patient. The methods further comprise optimizing the patient's therapy, selecting the patient for treatment, or treating the patient accordingly. In various aspects the biological samples are sections of a biopsy (e.g., a formalin fixed paraffin embedded biopsy). In other aspects the biomarkers are proteins and/or nucleic acids. In other aspects the biomarkers function in ErbB-mediated signal transduction.
1. A method of treating a human patient having lung cancer with a heregulin RNA in situ hybridization (RNA-ISH) score of 1+ or higher measured in a biological sample from the patient, the method comprising administering to the patient an effective amount of:
1) a first agent that is an anti-ErbB3 antibody, wherein the anti-ErbB3 antibody comprises CDRH1, CDRH2, and CDRH3 sequences comprising the amino acid sequences set forth in SEQ ID NO: 3 (CDRH1), SEQ ID NO: 4 (CDRH2), and SEQ ID NO: 5 (CDRH3), respectively, and CDRL1, CDRL2, and CDRL3 sequences comprising the amino acid sequences set forth in SEQ ID NO: 6 (CDRL1), SEQ ID NO: 7 (CDRL2), and SEQ ID NO: 8 (CDRL3), respectively; and
2) a second agent that is an anticancer agent, wherein said second anticancer agent is docetaxel or paclitaxel.
2. The method of claim 1 , wherein the RNA-ISH uses one or more nucleic acid probes that specifically hybridize to RNAs that encode each of the heregulin isoforms α, β1, β1b, β1c, β1d, β2, β2b, β3, β3b, γ, γ2, γ3, ndf43, ndf43b, and GGF2.
3. The method of claim 2 , wherein the one or more nucleic acid probes specifically hybridize to a nucleic acid comprising nucleotides 442-2977 of SEQ ID NO:42.
4. The method of claim 1 , wherein the anti-ErbB3 antibody comprises V H and V L amino acid sequences set forth in SEQ ID NO:1 and SEQ ID NO:2, respectively.
5. The method of claim 1 , wherein the sample is a microtome section of a biopsy.
6. The method of claim 5 , wherein the biopsy is a formalin fixed and paraffin embedded biopsy.
7. The method of claim 1 , wherein the sample was taken from the patient within 90 days prior to treating the patient.
8. The method of claim 1 , wherein the treatment produces at least one therapeutic effect selected from the group consisting of reduction in size of a tumor, reduction in number of metastatic lesions over time, complete response, partial response, stable disease, or a pathologic complete response.
9. The method of claim 1 , wherein the second anticancer agent is paclitaxel.
10. The method of claim 1 , wherein the second anticancer agent is docetaxel.
11. The method of claim 1 , wherein the lung cancer is NSCLC.
12. A method of treating a human patient having lung cancer comprising:
1) selecting a human lung cancer patient having a heregulin RNA in situ hybridization (RNA-ISH) score of 1+ or higher measured in a biological sample from the patient, and
2) administering to the patient (a) an effective amount of a first agent that is an anti-ErbB3 antibody, wherein the anti-ErbB3 antibody comprises CDRH1, CDRH2, and CDRH3 sequences comprising the amino acid sequences set forth in SEQ ID NO: 3 (CDRH1), SEQ ID NO: 4 (CDRH2), and SEQ ID NO: 5 (CDRH3), respectively, and CDRL1, CDRL2, and CDRL3 sequences comprising the amino acid sequences set forth in SEQ ID NO: 6 (CDRL1), SEQ ID NO: 7 (CDRL2), and SEQ ID NO: 8 (CDRL3), respectively; and (b) a second agent that is an anticancer agent, wherein said second anticancer a ent is docetaxel or aclitaxel.
13. The method of claim 12 , wherein the anti-ErbB3 antibody comprises V H and V L amino acid sequences set forth in SEQ ID NO:1 and SEQ ID NO:2, respectively.
14. The method of claim 12 , wherein the second anticancer agent a is paclitaxel.
15. The method of claim 12 , wherein the second anticancer agent is docetaxel.
16. The method of claim 12 , wherein the treatment produces at least one therapeutic effect selected from the group consisting of reduction in size of a tumor, reduction in number of metastatic lesions over time, complete response, partial response, stable disease, or a pathologic complete response.
17. The method of claim 12 , wherein the lung cancer is NSCLC.
18. A method of treating a human patient having lung cancer comprising:
1) selecting a human lung cancer patient having a heregulin RNA in situ hybridization (RNA-ISH) score of 1+ or higher measured in a biological sample from the patient, and
2) administering to the patient (a) an effective amount of a first agent that is an anti-ErbB3 antibody, wherein the anti-ErbB3 antibody comprises V H and V L amino acid sequences set forth in SEQ ID NO: 1 and SEQ ID NO:2, respectively; and (b) a second agent that is an anticancer agent, wherein said second anticancer agent is docetaxel or paclitaxel.
19. The method of claim 18 , wherein the second anticancer agent a is paclitaxel.
20. The method of claim 18 , wherein the second anticancer agent is docetaxel.
21. The method of claim 18 , wherein the treatment produces at least one therapeutic effect selected from the group consisting of reduction in size of a tumor, reduction in number of metastatic lesions over time, complete response, partial response, stable disease, or a pathologic complete response.
22. The method of claim 18 , wherein the lung cancer is NSCLC.
23. The method of claim 12 , wherein the RNA-ISH uses one or more nucleic acid probes, wherein the one or more nucleic acid probes specifically hybridize to a nucleic acid comprising nucleotides 442-2977 of SEQ ID NO:42.
24. The method of claim 18 , wherein the RNA-ISH uses one or more nucleic acid probes, wherein the one or more nucleic acid probes specifically hybridize to a nucleic acid comprising nucleotides 442-2977 of SEQ ID NO:42.