Method for preparing biological tissues for use in biological prostheses
View Patent ↗A method of treating a biological tissue for biological prostheses includes steps of fixation of the biological tissue via a fixing solution including glutaraldehyde and detoxification of the fixed biological tissue via treatment with a detoxifying solution. The detoxification step includes one or both of eliminating phospholipids via treatment with an elimination solution and a treatment with a detoxifying solution. The elimination solution includes 1,2-octanediol and ethanol. The detoxifying solution includes taurine or homocysteic acid.
1. A method of treating a biological tissue for use in a biological prosthesis, the method comprising the steps:
eliminating phospholipids from the biological tissue by treating the biological tissue with an elimination solution at a temperature of 15° C. to 65° C., the elimination solution including 1,2-octanediol at a concentration of 1% v/v to 25% v/v and ethanol at a concentration of 25% to 49% v/v in a buffer at a pH of 4 to 9;
fixing the biological tissue treated with the elimination solution by treating the biological tissue, the fixing solution including glutaraldehyde at a concentration of 0.10% v/v to 2.00% v/v in a buffer at a pH of 5 to 8; and
detoxifying the biological tissue treated with the fixing solution by treating the biological tissue with a detoxifying solution at a temperature above 20° C., the detoxifying solution including taurine or homocysteic acid in a buffer at a pH of 4 to 9.
2. The method according to claim 1 , wherein the elimination solution includes 1,2-octanediol at a concentration of 2% v/v to 10% v/v.
3. The method according to claim 1 , wherein the elimination solution includes ethanol at a concentration of 30% v/v to 45% v/v.
4. The method according to claim 1 , wherein the eliminating of phospholipids is at a temperature of 30° C. to 40° C.
5. The method according to claim 1 , wherein the eliminating of phospholipids is carried out for a time period in the range of 2 to 7 days.
6. The method according to claim 1 , wherein the eliminating of phospholipids is carried out for a time period in the range of 16 to 72 hours.
7. The method according to claim 1 , wherein the elimination solution has a pH of 5 to 8.
8. The method according to claim 1 , wherein the detoxifying the biological tissue occurs at a temperature above 30° C.
9. The method according to claim 1 , wherein the detoxifying the biological tissue occurs at a temperature of 30-50° C.
10. The method according to claim 1 , wherein the detoxifying the biological tissue occurs at a temperature of 40° C.
11. The method according to claim 1 , wherein the detoxifying solution includes taurine.
12. The method according to claim 1 , wherein the detoxifying solution has a pH of 5 to 8.
13. The method according to claim 1 , wherein the fixing solution contains glutaraldehyde at a concentration expressed of 0.30% v/v to 1.00% v/v.