Acoustic energy treatment using phase change couplant
Method and apparatus for transmitting acoustic energy to a sample for treatment by employing a phase change couplant at least adjacent a sample vessel. The phase change couplant may be solid before and after treatment, but liquid during treatment to improve acoustic energy transmission charactersitics.
1. An acoustic treatment device, comprising:
a vessel arranged to hold a sample to be treated with acoustic energy;
an acoustic energy source for providing acoustic energy to the sample while the sample is in the vessel and separated from the acoustic energy source, the acoustic energy having a focal zone located at the vessel; and
an acoustic coupling medium between the acoustic energy source and the vessel, the acoustic coupling medium including a phase changing couplant located adjacent an exterior of the vessel and being solid at room temperature, the phase changing couplant changing to a liquid at least in a region in contact with the vessel in response to exposure to acoustic energy used the treat the sample in the vessel and returning to a solid state after exposure of the phase changing couplant to acoustic energy is stopped.
2. The device of claim 1 , wherein the acoustic coupling medium includes a solid coupling material between the acoustic energy source and the phase changing couplant.
3. The device of claim 2 , wherein the solid coupling material is arranged to focus acoustic energy received from the acoustic energy source.
4. The device of claim 3 , wherein the acoustic energy source includes an acoustic transducer that is flat and generates a planar wave of acoustic energy received by the solid coupling material.
5. The device of claim 4 , further comprising a matching layer between the acoustic transducer and the solid coupling material.
6. The device of claim 1 , wherein the acoustic coupling medium includes a liquid coupling material between the acoustic energy source and the phase changing couplant.
7. The device of claim 6 , wherein the liquid coupling material is water.
8. The device of claim 7 , wherein the liquid coupling material is arranged to focus acoustic energy received from the acoustic energy source.
9. The device of claim 8 , wherein the acoustic energy source includes an acoustic transducer that is flat and generates a planar wave of acoustic energy received by the liquid coupling material.
10. The device of claim 9 , further comprising a matching layer between the acoustic transducer and the liquid coupling material.
11. The device of claim 6 , comprising a liquid impermeable membrane between the liquid coupling material and the phase changing couplant.
12. The device of claim 11 , wherein the impermeable membrane includes a silicone rubber or polymethylpentene (TPX) material.
13. The device of claim 1 , wherein the acoustic coupling medium defines a vessel holder arranged to support the vessel at a location at least partially in the focal zone of the acoustic energy.
14. The device of claim 1 , wherein the acoustic energy directed to the sample is sufficient to cause at least one of lysing, extraction, permeabilizing, stirring, catalyzing, degrading, fluidization, heating, particle breakdown, DNA shearing, or disruption of molecular bonds in the sample.
15. The device of claim 1 , wherein the acoustic energy source is spaced from and exterior to the vessel, and the acoustic energy comprises a frequency of between about 100 kilohertz and about 100 megahertz and a focal zone having a width of less than about 2 centimeters, and wherein at least a portion of the acoustic energy is adapted to propagate exterior to the vessel.
16. The device of claim 1 , wherein the acoustic energy source includes an acoustic transducer having a dome shape and arranged to generate focused acoustic energy to create the focal zone.
17. The device of claim 1 , wherein the sample has a volume of 10 microliters to 150 milliliters.