ONCOGENIC MODELS BASED ON DELIVERY AND USE OF THE CRISPR-CAS SYSTEMS, VECTORS AND COMPOSITIONS
The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are delivery systems and tissues or organ which are targeted as sites for delivery. Also provided are vectors and vector systems some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells to enable genome engineering in an organism to recapitulate the genetic complexity of disease or a condition and further interrogate gene function.
1 . A method of inducing a proliferative condition in an organism comprising:
isolating a first population of cells from the organism,
transducing the first population of cells with a non-naturally occurring or engineered composition comprising a vector system comprising one or more vectors comprising
I. a first regulatory element operably linked to a CRISPR-Cas system chimeric RNA (chiRNA) polynucleotide sequence, wherein the polynucleotide sequence comprises
(a) three or more guide sequences capable of hybridizing to three or more target sequences in genome of the organism,
(b) a tracr mate sequence, and
(c) a tracr sequence, and
II. a second regulatory element operably linked to an enzyme-coding sequence encoding a CRISPR enzyme comprising at least one or more nuclear localization sequences (NLSs),
wherein (a), (b) and (c) are arranged in a 5′ to 3′ orientation,
wherein components I and II are located on the same or different vectors of the system,
wherein when transcribed, the tracr mate sequence hybridizes to the tracr sequence and the guide sequence direct sequence-specific binding of CRISPR complexes to the target sequence,
wherein the CRISPR complex comprises the CRISPR enzyme complexed with (1) the guide sequence that is hybridizable to the target sequence, and (2) the tracr mate sequence that is hybridized to the tracr sequence,
wherein the CRISPR enzyme alters the genome of the first population of cells to obtain a second population of cells, and
transplanting the second population of cells into the organism thereby inducing the proliferative condition.
2 . A method of interrogating function of one or more genes in a proliferative condition in an organism comprising:
inducing the proliferative condition by a method comprising
isolating a first population of cells from the organism,
transducing the first population of cells with a non-naturally occurring or engineered composition comprising a vector system comprising one or more vectors comprising
I. a first regulatory element operably linked to a CRISPR-Cas system chiRNA polynucleotide sequence, wherein the polynucleotide sequence comprises
(a) three or more guide sequences capable of hybridizing to three or more target sequences in genome of the organism,
(b) a tracr mate sequence, and
(c) a tracr sequence, and
II. a second regulatory element operably linked to an enzyme-coding sequence encoding a CRISPR enzyme comprising zero or at least one or more NLSs,
wherein (a), (b) and (c) are arranged in a 5′ to 3′ orientation,
wherein components I and II are located on the same or different vectors of the system,
wherein when transcribed, the tracr mate sequence hybridizes to the tracr sequence and the guide sequence direct sequence-specific binding of CRISPR complexes to the target sequence,
wherein the CRISPR complex comprises the CRISPR enzyme complexed with (1) the guide sequence that is hybridizable to the target sequence, and (2) the tracr mate sequence that is hybridized to the tracr sequence,
wherein the CRISPR enzyme alters the genome of the first population of cells to obtain a second population of cells,
transplanting the second population of cells into the organism thereby inducing the proliferative condition; and
determining changes in expression of the one or more genes in the proliferative condition thereby interrogating the function of one or more genes.
3 . The method of claim 1 or 2 , wherein the proliferative condition is a cancer.
4 . The method of claim 3 , wherein the first population of cells is a population of stem cells.
5 . The method of claim 1 or 2 , wherein the proliferative condition is a myeloid malignancy.
6 . The method of claim 5 , wherein the first population of cells is a population of hematopoietic stem and progenitor cells.
7 . The method of claim 1 or 2 , wherein the vector system is a lentiviral vector system.
8 . The method of claim 7 , wherein the first regulatory element is a U6 promoter.
9 . The method of claim 7 , wherein the second regulatory element is an EFS promoter.
10 . The method of claim 1 or 2 , wherein the polynucleotide sequence comprises 4, 5, 6, 7, 8, 9 or 10 guide sequences.
11 . The method of claim 1 or 2 , wherein the target sequences are associated with tumor suppressor gene(s).
12 . A non-naturally occurring or engineered composition for generating an in vivo, in vitro or ex vivo proliferative condition comprising multiple genetic lesions in a population of cells comprising a lentiviral vector system comprising one or more lentiviral vectors comprising
I. a U6 promoter operably linked to a CRISPR-Cas system chiRNA polynucleotide sequence, wherein the polynucleotide sequence comprises
(a) three or more guide sequences capable of hybridizing to three or more target sequences in genome of the organism,
(b) a tracr mate sequence, and
(c) a tracr sequence, and
II. a EFS promoter operably linked to an enzyme-coding sequence encoding a CRISPR enzyme comprising zero or at least one or more NLSs,
wherein (a), (b) and (c) are arranged in a 5′ to 3′ orientation,
wherein components I and II are located on the same or different vectors of the system,
wherein when transcribed, the tracr mate sequence hybridizes to the tracr sequence and the guide sequence direct sequence-specific binding of CRISPR complexes to the target sequence, and
wherein the CRISPR complex comprises the CRISPR enzyme complexed with (1) the guide sequence that is hybridizable to the target sequence, and (2) the tracr mate sequence that is hybridized to the tracr sequence.
13 . The composition of claim 12 , wherein the proliferative condition is a cancer.
14 . The composition of claim 12 , wherein the population of cells is a population of stem cells.
15 . The composition of claim 12 , wherein the proliferative condition is a myeloid malignancy.
16 . The composition of claim 12 , wherein the population of cells is a population of hematopoietic stem and progenitor cells.
17 . The composition of claim 12 , wherein the polynucleotide sequence comprises 4, 5, 6, 7, 8, 9 or 10 guide sequences.
18 . The composition of claim 12 , wherein the target sequences are associated with tumor suppressor gene(s).