CRISPR-based compositions and methods of use
View Patent ↗This invention pertains to modified compositions for use in CRISPR systems, and their methods of use. In particular, length-modified and chemically-modified forms of crRNA and tracrRNA are described for use as a reconstituted guide RNA for interaction with Cas9 of CRIPSR systems. The resultant length-modified and chemically-modified forms of crRNA and tracrRNA are economical to produce and can be tailored to have unique properties relevant to their biochemical and biological activity in the context of the CRIPSR Cas9 endonuclease system.
1. An isolated tracrRNA selected from the group consisting of SEQ ID NOs.: 11-13, 17, 87-107, 109-118, 120-122, 126, 129, 131, 132, 134-137, 142, 144, 145, and 147-162, wherein the isolated tracrRNA is active in a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein endonuclease system.