Compositions to detect seasonal H1 influenza A virus nucleic acids
View Patent ↗Methods for detecting the presence or absence of the swine H1N1 influenza A virus, seasonal H1 influenza A virus and/or seasonal H3 influenza A virus nucleic acids in biological samples are disclosed. Compositions that are target-specific nucleic acid sequences and kits comprising target-specific nucleic acid oligomers for amplifying in vitro the swine H1N1 influenza A virus, seasonal H1 influenza A virus and/or seasonal H3 influenza A virus nucleic acid and detecting amplified nucleic acid sequences are disclosed.
1. A composition comprising:
at least first and second seasonal H1 influenza A-specific amplification primers, wherein the first primer comprises a H1 influenza A-specific sequence consisting of SEQ ID NO:72 and the second primer comprises a H1 influenza A-specific sequence consisting of SEQ ID:73; and
a seasonal H1 influenza A-specific oligonucleotide detection probe having a H1 influenza A-specific sequence of at least 18 contiguous nucleotides, wherein said detection probe is complementary to a H1 influenza A nucleic acid sequence that is amplifiable by the first and second primers, and wherein said detection probe comprises at least a first detectable label operably linked to said oligonucleotide detection probe.
2. The composition according to claim 1 , wherein at least one of said nucleic acid primers or said probe includes one or more nucleotides at the 5′ end that are not specific for H1 influenza A nucleic acid, and wherein said nucleic acid primers and said probe are no more than 50 nucleotides in length.
3. The composition according to claim 1 , wherein said first and second primers are SEQ ID NO:72 and SEQ ID NO:73,respectively and the H1 influenza A-specific sequence of said probe is SEQ ID NO:74.
4. A kit comprising the composition according to claim 3 .
5. The composition of claim 1 , wherein
said first and second primers are SEQ ID NO:72 and SEQ ID NO:73, respectively.
6. The composition of claim 5 , wherein the H1 influenza A-specific sequence of said probe is selected from the group consisting of SEQ ID NO:74, SEQ ID NO:75, and SEQ ID NO:76.
7. A kit comprising, in a suitable container, a composition according to claim 6 , and instructions for use of said composition in any assay for amplification of polynucleotides in a biological sample.
8. A kit comprising a composition according to claim 1 .
9. The kit according to claim 8 , wherein the kit further comprises instructions for use of said composition in any assay for amplification of polynucleotides in a biological sample.